Department of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
Department of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
J Mech Behav Biomed Mater. 2024 May;153:106493. doi: 10.1016/j.jmbbm.2024.106493. Epub 2024 Mar 2.
Elastomeric biocomposites based on poly(glycerol adipate urethane) and hydroxyapatite were fabricated for tissue regeneration. The poly(glycerol adipate urethane) (PGAU) elastomeric composite matrices were obtained by chemical crosslinking of the poly(glycerol adipate) prepolymer (pPGA) with diisocyanate derivative of L-lysine. Two series of composites varying in the amount of L-lysine diisocyanate ethyl ester (LDI) used as a crosslinking agent were manufactured. As a ceramic filler both unmodified and L-lysine surface-modified hydroxyapatite (HAP) particles were used. The novelty of our research consists in the manufactured elastomeric materials and characterization of their linear viscoelastic (LVE) properties. The LVE properties of the composites were investigated by means of dynamic thermomechanical analysis. Frequency sweep and amplitude sweep measurements were performed in shear mode. The influence of the crosslinking agent (LDI) amount, HAP content and surface modification of HAP on the LVE properties of the composites was determined based on the analysis of the master curves of storage (G') and loss (G″) moduli and of tanδ of the composites. Depending on the amount of LDI, HAP and surface modification, the materials differ in the values of rubber elasticity plateau modulus (G) and G' and G″ determined at selected shear frequencies and at the glassy state. G ranges from 278 kPa to 3.98 MPa, G' in the glassy state is within the range of 219 MPa-459 MPa. The G values of the PGAU-based composites are within the stiffness range of soft tissue. In view of the choice of HAP as the ceramic component and the G values, elastomeric composites have the potential to be used as filling materials in small bone defects (due to their mechanical similarity to osteoid) as well as materials for cartilage tissue regeneration.
基于聚（己二酸甘油酯）聚氨酯和羟基磷灰石的弹性体生物复合材料被用于组织再生。通过聚（己二酸甘油酯）预聚物（pPGA）与 L-赖氨酸二异氰酸酯衍生物的化学交联，获得了聚（己二酸甘油酯）弹性体复合基质（PGAU）。制造了两种系列的复合材料，其变化在于用作交联剂的 L-赖氨酸二异氰酸酯乙酯（LDI）的量。使用未改性和 L-赖氨酸表面改性的羟基磷灰石（HAP）颗粒作为陶瓷填料。我们研究的新颖之处在于制造的弹性体材料及其线性粘弹性（LVE）特性的表征。通过动态热机械分析研究了复合材料的 LVE 特性。在剪切模式下进行了频率扫描和振幅扫描测量。根据储能模量（G'）和损耗模量（G"）和复合材料的损耗角正切（tanδ）的主曲线分析，确定了交联剂（LDI）量、HAP 含量和 HAP 表面改性对复合材料 LVE 特性的影响。根据 LDI、HAP 和表面改性的量，材料在选定的剪切频率和玻璃态下确定的橡胶弹性平台模量（G）和 G'和 G"的值上有所不同。G 的范围从 278 kPa 到 3.98 MPa，玻璃态下的 G'在 219 MPa-459 MPa 的范围内。基于 PGAU 的复合材料的 G 值处于软组织的刚度范围内。鉴于选择 HAP 作为陶瓷成分和 G 值，弹性体复合材料有可能被用作小骨缺损的填充材料（由于它们与类骨质的机械相似性）以及软骨组织再生的材料。
