Department of Genetic Engineering and Biomedical Institute for convergence (BICS), Sungkyunkwan University, Suwon 16419, Republic of Korea.
Research and Business Foundation, Sungkyunkwan University, Suwon 16419, Republic of Korea.
J Ethnopharmacol. 2018 Jun 28;220:57-66. doi: 10.1016/j.jep.2018.03.037. Epub 2018 Mar 31.
Artemisia asiatica Nakai is a traditional herbal plant that has long been used in anti-inflammatory, anti-infective and skin protective remedies.
In this study, traditionally known skin-protective activity of Artemisia asiatica Nakai was examined with its ethanol extract (Aa-EE) under various photoaging conditions using skin-originated cells, and the underlying mechanism was also examined using various types of cells.
Effects of Aa-EE on cell viability, photocytotoxicity, and expression of matrix metalloproteinases (MMPs), cyclooxygenase (COX)-2, and moisturizing factors were measured in B16F10, HEK293, NIH3T3, and HaCaT cells under untreated and ultraviolet B (UVB)-irradiation conditions. Anti-melanogenic effect of Aa-EE was also examined by measuring both melanin content in B16F10 cells and tyrosinase activity. Anti-photoaging mechanism of Aa-EE was explored by determining the activation levels of signaling molecules by immunoblotting analysis.
Aa-EE protected HaCaT cells from UVB irradiation-induced death. Aa-EE increased the expression of a type 1 pro-collagen gene and decreased the expression of matrix metalloproteinases, and COX-2 in NIH3T3 cells induced by UVB. Aa-EE increased the expression of transglutamase-1, hyaluronic acid synthase (HAS)-2, and HAS-3 in HaCaT cells and decreased the production of melanin in α-melanocyte-stimulating hormone-stimulated B16F10 cells by suppressing tyrosinase activity and the expression of tyrosinase, microphthalmia-associated transcription factor, tyrosinase-related protein (TRP)-1 and TRP-2.
The results suggest that Aa-EE could be skin-protective remedy with anti-photoaging, anti-apoptotic, skin remodeling, moisturizing, and anti-melanogenesis properties.
青蒿是一种传统的草本植物，长期以来一直被用于抗炎、抗感染和皮肤保护疗法。
本研究采用皮肤源性细胞，在各种光老化条件下，对青蒿传统上已知的皮肤保护活性进行了研究，并利用各种类型的细胞对其潜在机制进行了研究。
在未经紫外线 B（UVB）照射和 UVB 照射条件下，用 B16F10、HEK293、NIH3T3 和 HaCaT 细胞测量青蒿乙醇提取物（Aa-EE）对细胞活力、光细胞毒性以及基质金属蛋白酶（MMPs）、环氧化酶（COX）-2 和保湿因子表达的影响。还通过测量 B16F10 细胞中的黑色素含量和酪氨酸酶活性来检查 Aa-EE 的抗黑色素生成作用。通过免疫印迹分析确定信号分子的激活水平，探讨 Aa-EE 的抗光老化机制。
Aa-EE 可保护 HaCaT 细胞免受 UVB 照射诱导的死亡。Aa-EE 增加了 NIH3T3 细胞中由 UVB 诱导的Ⅰ型前胶原基因的表达，降低了 MMPs 和 COX-2 的表达。Aa-EE 增加了 HaCaT 细胞中转谷氨酰胺酶-1、透明质酸合酶（HAS）-2 和 HAS-3 的表达，并通过抑制酪氨酸酶活性和酪氨酸酶、小眼畸形相关转录因子、酪氨酸酶相关蛋白（TRP）-1 和 TRP-2 的表达，减少了α-促黑素细胞刺激素刺激的 B16F10 细胞中黑色素的产生。
结果表明，Aa-EE 可能是一种具有抗光老化、抗凋亡、皮肤重塑、保湿和抗黑色素生成作用的皮肤保护疗法。
