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用葡萄球菌蛋白A免疫酶法检测狂犬病抗体的微量方法(作者译)

[Micromethod for rabies antibody detection by immunoenzymatic assay with Staphylococcus protein A (author's transl)].

作者信息

Atanasiu P, Perrin P

出版信息

Ann Microbiol (Paris). 1979 Feb-Mar;130(2):257-68.

PMID:384866
Abstract

The rabies virus and its glycoprotein are used as antigens in a microimmunoenzymatic assay (IEA). Human rabies antibodies are detected and titrated using anti-human antibodies and staphylococcus protein A conjugated to peroxidase. Several mammal antirabies sera are tested by this method with protein A. The results are compared to those obtained by seroneutralization in mice, which is the standard method for rabies antibody detection.

摘要

狂犬病病毒及其糖蛋白被用作微免疫酶测定(IEA)中的抗原。使用抗人抗体和与过氧化物酶结合的葡萄球菌蛋白A来检测和滴定人狂犬病抗体。用这种方法用蛋白A检测几种哺乳动物抗狂犬病血清。将结果与通过小鼠血清中和法获得的结果进行比较,小鼠血清中和法是狂犬病抗体检测的标准方法。

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