Atanasiu P, Perrin P, Delagneau J F
Dev Biol Stand. 1980;46:207-15.
The rabies antigen quantitation reported here is based on the principle of an enzyme immuno micro assay (EIA) using antigen coated polystyrene microtiter plates. In a first step antibodies of known specificity are partially blocked by the antigen to be titrated; in a second step the free remaining antibodies are determined by EIA. Antirabies vaccines, purified virus or rabies glycoprotein were assayed by that micro-method in comparison with the double neutralization test in tissue culture. Moreover, we report results obtained by EIA on the rate of antigen bonding to a solid carrier in order to prepare immunoadsorbents and the usefulness of EIA to monitor specific immunoglobulin elution.
本文报道的狂犬病抗原定量是基于酶免疫微量测定（EIA）原理，使用抗原包被的聚苯乙烯微量滴定板。第一步，已知特异性的抗体被待滴定的抗原部分阻断；第二步，通过EIA测定剩余的游离抗体。采用该微量方法对抗狂犬病疫苗、纯化病毒或狂犬病糖蛋白进行了检测，并与组织培养中的双中和试验进行了比较。此外，我们还报告了通过EIA获得的关于抗原与固体载体结合率的结果，以便制备免疫吸附剂，以及EIA在监测特异性免疫球蛋白洗脱方面的实用性。
