用于检测鸡血清中传染性支气管炎病毒抗体的微孔板酶联免疫吸附测定（ELISA）的抗原要求和特异性
Antigen requirements and specificity of a microplate enzyme-linked immunosorbent assay (ELISA) for detecting infectious bronchitis viral antibodies in chicken serum.
作者信息
Soula A, Moreau Y
出版信息
Arch Virol. 1981;67(4):283-95. doi: 10.1007/BF01314832.
The conditions of a rapid, indirect-enzyme linked immunosorbent assay for Infectious Bronchitis Virus (IBV) antibodies have been established. Optimal sensitivity was obtained using 10 micrograms/ml protein concentration of the Mass 41 strain purified from infected allantoic fluid. Specificity was demonstrated with Newcastle Disease Virus (NDV) antigen-antibody system. Negligible crossreactions were observed. After bromelain or lipase treatment IBV had an ELISA reactivity similar to untreated particles suggesting that peripheral constituents of IBV play a minor role when whole virus is absorbed on solid phase. The method offers a simple and specific antibody assay which could be used for the laboratory diagnosis of avian infectious bronchitis.
已建立了一种用于检测传染性支气管炎病毒（IBV）抗体的快速间接酶联免疫吸附测定方法。使用从感染的尿囊液中纯化的Mass 41株，蛋白质浓度为10微克/毫升时可获得最佳灵敏度。用新城疫病毒（NDV）抗原 - 抗体系统证明了其特异性。观察到交叉反应可忽略不计。经菠萝蛋白酶或脂肪酶处理后，IBV的ELISA反应性与未处理的颗粒相似，这表明当全病毒吸附在固相上时，IBV的外周成分起的作用较小。该方法提供了一种简单且特异的抗体检测方法，可用于禽传染性支气管炎的实验室诊断。
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