Dimmick R L, Wolochow H, Chatigny M A
Appl Environ Microbiol. 1979 May;37(5):924-7. doi: 10.1128/aem.37.5.924-927.1979.
Serratia marcescens incubated for 8 h at 31 degrees C in a chemically defined medium contained in shake flasks was aerosolized into rotating-drum aerosol chambers at 30 degrees C and saturated humidity. Cells furnished tryptone (Difco) and glycerol just before aerosolization increased (in viable numbers and countable cells) almost twofold within 1 to 2 h after becoming airborne, whereas cells not furnished additional tryptone decreased in viable numbers at a faster rate than the number of particles removed by gravitational settling. Limited tests with a Coulter Counter showed that cell volume changes occurred in growing cells that did not occur in the nongrowing population.
将粘质沙雷氏菌在摇瓶中含有的化学成分确定的培养基中于31℃培养8小时后,在30℃和饱和湿度条件下雾化到转鼓式气溶胶室中。雾化前添加胰蛋白胨(Difco)和甘油的细胞,在变为空气传播状态后的1至2小时内,活细胞数量和可计数细胞数量几乎增加了两倍,而未添加额外胰蛋白胨的细胞,其活细胞数量下降的速度比因重力沉降而去除的颗粒数量更快。使用库尔特计数器进行的有限测试表明,生长中的细胞会发生细胞体积变化,而非生长群体中的细胞则不会。