College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang Province, China.
Hangzhou Biocom Co., Ltd, Hangzhou 310014, Zhejiang Province, China.
Bioresour Technol. 2024 May;399:130591. doi: 10.1016/j.biortech.2024.130591. Epub 2024 Mar 13.
Malachite Green (MG) is a widely used industrial dye that is hazardous to health. Herein, the decolourisation and detoxification of MG were achieved using the engineered Saccharomyces cerevisiae expressing novel thermostable laccase lcc1 from Trametes trogii. The engineered strain RCL produced a high laccase activity of 121.83 U L. Lcc1 was stable at temperatures ranging from 20 ℃ to 60 ℃ and showed a high tolerance to organic solvents. Moreover, Lcc1 could decolorize different kinds of dyes (azo, anthraquinone and triphenylmethane), among which, the decolorization ability of MG is the highest, reaching 95.10 %, and the decolorization rate of other triphenylmethane dyes also over 50 %. The RCL decolorized about 95 % of 50 mg L of MG dye in 10 h at 30 ℃. The MG degradation products were analyzed. The industrial application potential of the RCL was evaluated by treating industrial wastewater and the decolourisation rates were over 90 %.
孔雀石绿（MG）是一种广泛使用的工业染料，对健康有害。本文采用表达新型耐热漆酶 Lcc1 的工程酿酒酵母 Saccharomyces cerevisiae 实现了 MG 的脱色和解毒。工程菌 RCL 产生的漆酶活性高达 121.83 U L。Lcc1 在 20℃至 60℃的温度范围内稳定，对有机溶剂有很高的耐受性。此外，Lcc1 可以对不同类型的染料（偶氮、蒽醌和三苯甲烷）进行脱色，其中 MG 的脱色能力最高，达到 95.10%，其他三苯甲烷染料的脱色率也超过 50%。RCL 在 30℃下 10 小时内可将 50mg L 的 MG 染料脱色约 95%。分析了 MG 的降解产物。通过处理工业废水评估了 RCL 的工业应用潜力，脱色率超过 90%。
