Khanna Navya, Girija A S Smiline, Priyadharsini J Vijayashree
Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, P.H.Road, Chennai, Tamilnadu, 600077, India.
Heliyon. 2024 Mar 3;10(5):e27020. doi: 10.1016/j.heliyon.2024.e27020. eCollection 2024 Mar 15.
associated biofilm formation in enhances the virulence in concert with the high propensity of drug resistance. This research is thus aimed to characterize the gene molecularly and to examine its co-occurrence with MDR.
MDR strains of (N = 73) were selected to detect the formation of biofilms. Genomic DNA was extracted further and screened for followed by amplicon sequencing from the representative strains. Frequency of its distribution in different groups of drug resistant strains at a significant -value of <0.05 was further checked.
The biofilm assay showed high, low and negative biofilm formers in 58.9%, 31.5% and 0.9% of the strains respectively. The gene was detected in 14 strains of MDR (19.17%). Co-occurrence of gene was seen in all the strains that showed resistance to β-lactam inhibitors, cephems, carbapenems, fluoroquinolones and folates followed by 96% for the aminoglycosides and 25% in the efflux pump groups.
The study findings showed the occurrence of biofilms associated with in MDR strains. The results also suggest to track its role in varying the pattern of drug resistance with further experimentation.
[细菌名称]中相关生物膜的形成与高耐药性协同增强了毒力。因此，本研究旨在对[基因名称]进行分子特征分析，并检测其与多重耐药（MDR）的共现情况。
选择[细菌名称]的MDR菌株（N = 73）检测生物膜的形成。进一步提取基因组DNA并筛选[基因名称]，随后对代表性菌株进行扩增子测序。进一步检查其在不同耐药菌株组中分布频率的显著P值<0.05。
生物膜检测显示，分别有58.9%、31.5%和0.9%的菌株为高、低和阴性生物膜形成者。在14株MDR[细菌名称]中检测到[基因名称]（19.17%）。在所有对β-内酰胺酶抑制剂、头孢菌素、碳青霉烯类、氟喹诺酮类和叶酸耐药的菌株中均观察到[基因名称]的共现，其次氨基糖苷类为96%，外排泵组为25%。
研究结果显示MDR[细菌名称]菌株中存在与[细菌名称]相关的生物膜。结果还表明，需通过进一步实验追踪其在改变耐药模式中的作用。
