Peng Tao, Ke Zeyu, Wu Hao, He Jun, Sun Yue, Zhang Shuhe, Gao Qian, Shao Meng, Zhang Shengzhao, Zhong Zhensheng, Lu FengYa, Zhou Jinhua
School of Biomedical Engineering, Anhui Medical University, Hefei 230032, China.
College of Life Science, Anhui Medical University, Hefei 230032, China.
Biomed Opt Express. 2024 Feb 21;15(3):1785-1797. doi: 10.1364/BOE.514887. eCollection 2024 Mar 1.
Point-of-care testing (POCT) plays an increasingly important role in biomedical research and health care. Quantitative phase microscopes (QPMs) with good contrast, no invasion, no labeling, high speed and automation could be effectively applied for POCT. However, most QPMs are fixed on the optical platform with bulky size, lack of timeliness, which remained challenging in POCT solutions. In this paper, we proposed a plug-and-play QPM with multimode imaging based on the quantitative differential phase contrast (qDPC) method. The system employs a programmable LED array as the light source and uses the GPU to accelerate the calculation, which can realize multi-contrast imaging with six modes. Accurate phase measurement and real-time phase imaging are implemented by the proposed qDPC algorithms for quantitative phase targets and biomedical samples. A 3D electric control platform is designed for mechanical control of field of view and focusing without manual operations. The experimental results verify the robustness and high performance of the setup. Even a rookie could finish the POCT scheme for biomedical applications at the scene using the QPM with a compact size of 140 × 165 × 250 mm.
即时检验（POCT）在生物医学研究和医疗保健中发挥着越来越重要的作用。具有良好对比度、无侵入性、无需标记、高速且自动化的定量相显微镜（QPM）可有效应用于即时检验。然而，大多数QPM固定在光学平台上，体积庞大，缺乏及时性，这在即时检验解决方案中仍然具有挑战性。在本文中，我们提出了一种基于定量微分相衬（qDPC）方法的即插即用多模式成像QPM。该系统采用可编程LED阵列作为光源，并使用GPU加速计算，可实现六种模式的多对比度成像。通过所提出的qDPC算法对定量相目标和生物医学样本进行精确的相位测量和实时相位成像。设计了一个三维电控平台，用于在无需手动操作的情况下对视野和聚焦进行机械控制。实验结果验证了该装置的稳健性和高性能。即使是新手也可以在现场使用尺寸紧凑（140×165×250毫米）的QPM完成生物医学应用的即时检验方案。
