成体人类脑室下区神经干细胞的特性与本质
Identity and nature of neural stem cells in the adult human subventricular zone.
作者信息
Baig Salma, Nadaf Javad, Allache Redouane, Le Phuong U, Luo Michael, Djedid Annisa, Nkili-Meyong Andriniaina, Safisamghabadi Maryam, Prat Alex, Antel Jack, Guiot Marie-Christine, Petrecca Kevin
机构信息
Department of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital McGill University, 3801 University Avenue, Montreal QC H3A2B4, Canada.
Neuroimmunology Research Lab, Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, QC H2X0A9, Canada.
出版信息
iScience. 2024 Feb 27;27(4):109342. doi: 10.1016/j.isci.2024.109342. eCollection 2024 Apr 19.
The existence of neural stem cells (NSCs) in adult human brain neurogenic regions remains unresolved. To address this, we created a cell atlas of the adult human subventricular zone (SVZ) derived from fresh neurosurgical samples using single-cell transcriptomics. We discovered 2 adult radial glia (RG)-like populations, aRG1 and aRG2. aRG1 shared features with fetal early RG (eRG) and aRG2 were transcriptomically similar to fetal outer RG (oRG). We also captured early neuronal and oligodendrocytic NSC states. We found that the biological programs driven by their transcriptomes support their roles as early lineage NSCs. Finally, we show that these NSCs have the potential to transition between states and along lineage trajectories. These data reveal that multipotent NSCs reside in the adult human SVZ.
成人大脑海马神经发生区域中神经干细胞（NSCs）的存在仍未得到解决。为了解决这个问题，我们使用单细胞转录组学技术，从新鲜的神经外科手术样本中创建了成人人类脑室下区（SVZ）的细胞图谱。我们发现了2个成人放射状胶质细胞（RG）样群体，即aRG1和aRG2。aRG1与胎儿早期RG（eRG）具有共同特征，而aRG2在转录组上与胎儿外侧RG（oRG）相似。我们还捕获了早期神经元和少突胶质细胞NSC状态。我们发现，由它们的转录组驱动的生物学程序支持它们作为早期谱系NSCs的作用。最后，我们表明这些NSCs有在不同状态之间以及沿着谱系轨迹转变的潜力。这些数据揭示了多能NSCs存在于成人人类SVZ中。
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