Wang YaXuan, Wang Jinfeng, Liu Yang, Wang XiaoLin, Ren MingHua
Department of Urology, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
Department of Urology, Nantong Tumor Hospital, Nantong, 226361, China.
Heliyon. 2024 Feb 28;10(6):e27184. doi: 10.1016/j.heliyon.2024.e27184. eCollection 2024 Mar 30.
Endoplasmic reticulum (ER) stress-related genes are closely related to the occurrence, development, and immunotherapy response of tumors. This study provides a comprehensive assessment of HSPA5 from a pan-cancer perspective using multi-omics data. We analyzed the function of HSPA5 in multiple tumor types using multiple databases. Finally, immunohistochemistry was used to examine the relationship between HSPA5 expression in tissue microarrays from 100 patients with bladder cancer and the prognosis of patients with bladder cancer. Using the TCGA database, we were able to determine that HSPA5 is significantly elevated in a number of common malignancies and is linked with a bad prognosis. Cox regression analysis showed that the high expression of HSPA5 was correlated with OS, progression free survival (PFS), disease free survival (DFS), and disease special survival (DSS) of adrenocortical carcinoma (ACC). In addition, we discovered significant disparities in HSPA5 methylation and phosphorylation levels between various malignancies and normal tissues. HSPA5 expression was significantly correlated with the levels of infiltrating cells and immune checkpoint genes. HSPA5 is highly expressed in bladder cancer and patients with high HSPA5 expression have a poor prognosis. Our study provides a basis for further understanding of the role of ER stress-related gene HSPA5 in different tumor genesis and development. HSPA5 has also been shown to be a prognostic biomarker for bladder cancer patients.
内质网（ER）应激相关基因与肿瘤的发生、发展及免疫治疗反应密切相关。本研究利用多组学数据从泛癌角度对HSPA5进行了全面评估。我们使用多个数据库分析了HSPA5在多种肿瘤类型中的功能。最后，采用免疫组织化学方法检测了100例膀胱癌患者组织芯片中HSPA5表达与膀胱癌患者预后的关系。利用TCGA数据库，我们能够确定HSPA5在多种常见恶性肿瘤中显著升高，并与不良预后相关。Cox回归分析表明，HSPA5高表达与肾上腺皮质癌（ACC）的总生存期（OS）、无进展生存期（PFS）、无病生存期（DFS）和疾病特异性生存期（DSS）相关。此外，我们发现不同恶性肿瘤与正常组织之间HSPA5甲基化和磷酸化水平存在显著差异。HSPA5表达与浸润细胞水平和免疫检查点基因显著相关。HSPA5在膀胱癌中高表达，HSPA5高表达的患者预后较差。我们的研究为进一步了解内质网应激相关基因HSPA5在不同肿瘤发生发展中的作用提供了依据。HSPA5也已被证明是膀胱癌患者的预后生物标志物。
