Center of Reproductive Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, Fujian, China.
Department of Pathology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, Fujian, China.
Genes Immun. 2024 Apr;25(2):149-157. doi: 10.1038/s41435-024-00268-5. Epub 2024 Mar 18.
Repeated implantation failure (RIF) is one of the most prominent problems in the field of assisted reproduction. Neu5Gc on the surface of decidual macrophages (dMΦ) leads to different activation patterns of dMΦ, which affects embryo implantation and development. Cmah (Neu5Gc-deficient) mice induced to produce anti-Neu5Gc antibodies in vivo were given a special diet rich in Neu5Gc and their fertility was monitored. The long-term diet rich in Neu5Gc induced the decrease of endometrial receptivity of female mice. The pregnancy rate of female mice fed the normal diet was 63.6% (n = 11) and the average number of embryos was 9.571 ± 1.272, while the pregnancy rate of female mice fed the diet rich in Neu5Gc was 36.4% (n = 11) and the average number of embryos in pregnant mice was 5.750 ± 3.304. The intake of Neu5Gc and the production of anti-Neu5Gc antibody led to M1 polarization of endometrial dMΦ and abnormal embryo implantation.
反复着床失败（RIF）是辅助生殖领域中最突出的问题之一。 蜕膜巨噬细胞（dMΦ）表面的Neu5Gc 导致 dMΦ 呈现不同的激活模式，从而影响胚胎着床和发育。 在体内诱导 Cmah（Neu5Gc 缺陷）小鼠产生抗-Neu5Gc 抗体，并用富含 Neu5Gc 的特殊饮食进行喂养，并监测其生育能力。 长期富含 Neu5Gc 的饮食会降低雌性小鼠的子宫内膜容受性。 正常饮食喂养的雌性小鼠的妊娠率为 63.6%（n=11），平均胚胎数为 9.571±1.272，而富含 Neu5Gc 饮食喂养的雌性小鼠的妊娠率为 36.4%（n=11），怀孕小鼠的平均胚胎数为 5.750±3.304。Neu5Gc 的摄入和抗-Neu5Gc 抗体的产生导致了子宫内膜 dMΦ 的 M1 极化和胚胎着床异常。
