Bonaventure J, Nigam V N, Brailovsky C A
Cancer Immunol Immunother. 1985;20(2):167-74. doi: 10.1007/BF00205684.
The nature of spleen cells in Fischer rats bearing a large size (greater than 1 cm diameter) mammary adenocarcinoma 13762A (MAC) which block the immunostimulating capacities of MTP2 (a synthetic immunomodulator) and suppress proliferation in vitro of splenic T and B lymphocytes by their respective mitogens was investigated. Splenic macrophages were recognized as the suppressor cells by (a) restoration of mitogenic responses by depletion of macrophages from spleen cell suspensions and (b) continued suppressor activity in spleen cell suspensions of tumor bearers devoid of viable T lymphocytes. Macrophage contact with T lymphocytes was required for the inhibition of T lymphocyte proliferation by concanavalin A as shown by (a) the absence of suppressor activity in supernatants derived from cultured suppressor macrophages, (b) lowering of the suppressor activity of intact macrophages after treatment with neuraminidase, (c) lowering of the suppressor activity of macrophages by addition of red cells to spleen cultures of tumor bearers indicating red cell interference with macrophage-T cell interaction and (d) lack of inhibiting action of suppressor macrophages on allogenic T lymphocyte proliferation showing macrophage T cell recognition for suppression. Animals bearing a large size tumor exhibited spleen hypertrophy and an increase in macrophage: lymphocyte ratio and a decrease in red cell: lymphocyte ratio. Splenic macrophages did not appear to be implicated in blocking antitumor immunity induction since (a) suppressor macrophages were absent in spleens during the inductive phase of the immune response and (b) MAC implanted in allogenic Wistar rats grew to about 2 cm diameter, induced splenic suppressor macrophages but the tumor was later rejected by the animals. Collectively the results suggest that suppressor macrophages are the result of increasing tumor volume rather than its cause.
对患有大尺寸（直径大于1厘米）乳腺腺癌13762A（MAC）的Fischer大鼠的脾细胞性质进行了研究，这些脾细胞会阻断MTP2（一种合成免疫调节剂）的免疫刺激能力，并通过其各自的丝裂原抑制脾T淋巴细胞和B淋巴细胞的体外增殖。脾巨噬细胞被认为是抑制细胞，依据如下：（a）通过从脾细胞悬液中去除巨噬细胞可恢复有丝分裂反应；（b）在缺乏活T淋巴细胞的荷瘤动物的脾细胞悬液中持续存在抑制活性。如以下所示，巨噬细胞与T淋巴细胞的接触是伴刀豆球蛋白A抑制T淋巴细胞增殖所必需的：（a）培养的抑制性巨噬细胞的上清液中不存在抑制活性；（b）用神经氨酸酶处理后，完整巨噬细胞的抑制活性降低；（c）在荷瘤动物的脾培养物中加入红细胞可降低巨噬细胞的抑制活性，表明红细胞干扰巨噬细胞与T细胞的相互作用；（d）抑制性巨噬细胞对同种异体T淋巴细胞增殖缺乏抑制作用，表明巨噬细胞对T细胞的识别用于抑制。患有大尺寸肿瘤的动物表现出脾肿大，巨噬细胞与淋巴细胞的比例增加，红细胞与淋巴细胞的比例降低。脾巨噬细胞似乎与抗肿瘤免疫诱导的阻断无关，因为：（a）在免疫反应的诱导阶段，脾中不存在抑制性巨噬细胞；（b）植入同种异体Wistar大鼠的MAC生长至约2厘米直径，诱导出脾抑制性巨噬细胞，但肿瘤后来被动物排斥。总体而言，结果表明抑制性巨噬细胞是肿瘤体积增加的结果而非原因。
