Rudczynski A B, Mortensen R F
J Natl Cancer Inst. 1978 Jan;60(1):205-11. doi: 10.1093/jnci/60.1.205.
Suppressor cell activity was present in the glass-adherent fraction of spleen cells from C3H mice bearing murine mammary tumor virus-induced mammary tumors. These cells effectively suppressed the blastogenic response of syngeneic normal lymphocytes to concanavalin A (Con A). Suppression by the spleen cells from mammary tumor-bearing mice was not dependent on DNA synthesis. Removal of the suppressor cells from spleen cell suspensions of tumor-bearing mice was not dependent on DNA synthesis. Removal of the suppressor cells from spleen cell suspensions of tumor-bearing animals (TBA) by passage of the cells on glass wool columns increased the Con A response of the remaining cells by fourfold to eightfold. Characterization of the suppressor population indicated that the cells were also adherent to nylon wool but not to plastic and contained a significantly increased proportion of surface immunoglobulin-bearing and complement receptor-bearing lymphocytes. Depletion of macrophages and T-cells did not remove the suppressive activity from the spleens of the TBA. The results were consistent with the identification of the suppressor cell as a B-cell.
在携带鼠乳腺肿瘤病毒诱导的乳腺肿瘤的C3H小鼠脾脏细胞的玻璃黏附部分中存在抑制细胞活性。这些细胞有效地抑制了同基因正常淋巴细胞对刀豆球蛋白A（Con A）的增殖反应。来自荷乳腺肿瘤小鼠的脾脏细胞的抑制作用不依赖于DNA合成。从荷瘤小鼠脾脏细胞悬液中去除抑制细胞不依赖于DNA合成。通过将细胞通过玻璃棉柱，从荷瘤动物（TBA）的脾脏细胞悬液中去除抑制细胞，可使剩余细胞的Con A反应增加4至8倍。对抑制细胞群体的表征表明，这些细胞也黏附于尼龙棉，但不黏附于塑料，并且含有显著增加比例的表面带有免疫球蛋白和补体受体的淋巴细胞。巨噬细胞和T细胞的耗竭并未消除TBA脾脏中的抑制活性。这些结果与将抑制细胞鉴定为B细胞一致。
