Protective Effect of Extract Fermented by Four Different Strains on HO Induced Oxidative Stress in Skin Fibroblasts.

PURPOSE

To produce extract as a cosmetic ingredient through fermentation.

METHODS

We first compared the total sugar content, polysaccharide content, reducing sugar content, total phenolic content, total saponin content, DPPH free radical, ABTS free radical, hydroxyl free radical scavenging ability and ferric reducing antioxidant power (FRAP) of Panax notoginseng fermented extract (FE) and unfermented extract (WE). Their potential correlations were analyzed by Pearson's correlation analysis. Then, the oxidative stress model of HO-induced MSFs was used to evaluate the effects of different FE on MSF viability, reactive oxygen species (ROS), malondialdehyde (MDA), and the activities of catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) to explore their protective effects on MSFs subjected to HO-induced cellular oxidative damage. Finally, their safety and stability were evaluated by using the red blood cell (RBC) test and hen's egg test-chorioallantoic membrane (HET-CAM) assay, and changes in pH and content of soluble solids, respectively.

RESULTS

Compared with WE, FE has more active substances and stronger antioxidant capacity. In addition, FE has a protective effect on HO-induced oxidative stress in MSFs with appropriate safety and stability.

CONCLUSION

FE has broad application prospects in the field of cosmetics.