Exploring the Protective and Reparative Mechanisms of Polysaccharides Against HO-Induced Oxidative Stress in Human Skin Fibroblasts.

BACKGROUND

() is one of China's traditional medicinal materials. polysaccharide has a wide range of promising pharmacological applications. However, there are many kinds of and they contain different kinds of polysaccharides. The biological mechanism through which polysaccharides (GLP) is able to protect human skin fibroblasts (HSFs) from HO-induced oxidative damage is still unclear.

METHODS

Six polysaccharides were obtained from to evaluate their free radical scavenging ability (DPPH free radical, ABTS free radical, hydroxyl-free radical, superoxide anion-free radical) in vitro, and their protective and reparative effects on oxidative damage induced by HO in human skin fibroblasts. One polysaccharide was selected to detect oxidative damage markers and gene expression in the Keap1-Nrf2/ARE signaling pathway in HSFs.

RESULTS

All six polysaccharides showed the ability to scavenge free radicals and enhance the tolerance of human skin fibroblasts to H2O2 damage. Among them, GLP1 was selected and separated into two components (GLP1I and GLP1II). The results showed that GLP1, GLP1I and GLPII could significantly reduce the levels of reactive oxygen species (ROS) and malondialdehyde (MDA). The protective effect of GLP1II was stronger than that of positive control vitamin C. In addition, GLP1, GLP1I and GLP1II could significantly increase the levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). And GLP1I works best in both ways. Meanwhile, Nrf2, a key regulator of keAP1-NRF2/ARE signaling pathway, was activated, while Keap1, a negative regulator, was inhibited, thus promoting the expression of downstream antioxidant enzyme genes (GSTs, GCLs, Nqo1, and Ho-1).

CONCLUSION

The results showed that GLP could protect human skin fibroblasts from oxidative damage caused by HO peroxide by enhancing enzyme activity and activating Keap1-Nrf2/ARE signaling pathway. GLP will act as a natural antioxidant to protect the skin from oxidative stress damage.