White E L, Bus J S, Heck H D
Biomed Mass Spectrom. 1979 Apr;6(4):169-72. doi: 10.1002/bms.1200060408.
A quantitative method is reported for the determination of n-hexane, 2-hexanone and 2,5-hexanedione in biological tissues by stable isotope dilution using gas chromatography mass spectrometry. n-[2H14]Hexane, 2-[2H5]hexanone and 2,5-[2H10]hexanedione are used as the isotopic diluents. After tissue homogenization and extraction of the three compounds in the presence of the deuterated internal standards, analysis is carried out in a single gas chromatographic injection by selected ion monitoring. Principal advantages of the technique are the ease of sample handling, rapidity of analysis and low limits of detection. The methodology is used to determine the organ distribution, pharmacokinetics and metabolism of n-hexane to 2-hexanone and 2,5-hexanedione in rats following inhalation exposure.
报道了一种采用气相色谱-质谱联用的稳定同位素稀释法定量测定生物组织中正己烷、2-己酮和2,5-己二酮的方法。使用正-[2H14]己烷、2-[2H5]己酮和2,5-[2H10]己二酮作为同位素稀释剂。在氘代内标存在的情况下对组织进行匀浆并提取这三种化合物后，通过选择离子监测在一次气相色谱进样中进行分析。该技术的主要优点是样品处理简便、分析速度快且检测限低。该方法用于测定大鼠吸入暴露后正己烷向2-己酮和2,5-己二酮的器官分布、药代动力学和代谢情况。
