Fedtke N, Bolt H M
Institut für Arbeitsphysiologie, Universität Dortmund, Federal Republic of Germany.
Biomed Environ Mass Spectrom. 1987 Oct;14(10):563-72. doi: 10.1002/bms.1200141006.
Male Wistar rats were exposed to 2000 ppm n-hexane or were treated with a single dose of 2,5-hexanedione (200 mg kg-1). Analysis of the urine collected after both treatments revealed the formation of 4,5-dihydroxy-2-hexanone via 5-hydroxy-2-hexanone or 2,5-hexanedione, respectively. Identification of this new n-hexane metabolite included enzymatic hydrolysis of the excreted glucuronide, derivatization of the keto group with O-methylhydroxylamine, and subsequent GC-MS analysis. The chemical structure derived from the mass spectra obtained was confirmed by further analysis of the methoxime-TMS derivatives and of deuterated 4,5-dihydroxy-2-hexanone excreted after treating rats with 2,5-[2H10]-hexanedione. 4,5-Dihydroxy-2-hexanone is proposed to be identical with a urinary constituent that is excreted by rats after n-hexane exposure and is converted to 2,5-dimethylfuran or 2,5-hexanedione, respectively, depending on the conditions of urine treatment prior to GC-MS analysis.
将雄性Wistar大鼠暴露于2000 ppm的正己烷中，或用单剂量的2,5 - 己二酮（200 mg kg-1）进行处理。对两种处理后收集的尿液进行分析发现，分别通过5 - 羟基 - 2 - 己酮或2,5 - 己二酮形成了4,5 - 二羟基 - 2 - 己酮。这种新的正己烷代谢物鉴定包括对排泄的葡糖醛酸化物进行酶促水解、用O - 甲基羟胺对酮基进行衍生化，以及随后的气相色谱 - 质谱分析。通过对甲肟 - TMS衍生物和用2,5 - [2H10] - 己二酮处理大鼠后排泄的氘代4,5 - 二羟基 - 2 - 己酮进行进一步分析，确认了从获得的质谱图得出的化学结构。有人提出，4,5 - 二羟基 - 2 - 己酮与大鼠在暴露于正己烷后排泄的一种尿液成分相同，并且根据气相色谱 - 质谱分析之前尿液处理的条件，分别转化为2,5 - 二甲基呋喃或2,5 - 己二酮。
