Reynolds P E, Brown D F
FEBS Lett. 1985 Nov 11;192(1):28-32. doi: 10.1016/0014-5793(85)80036-3.
Methicillin-resistant clinical isolates of Staphylococcus aureus are intrinsically resistant to beta-lactam antibiotics in that the resistance mechanism is unrelated to the possession of beta-lactamases. We have demonstrated that a new, high-molecular-mass penicillin-binding protein (PBP) is present in these strains with a low affinity for beta-lactams and that its amount is regulated by the growth conditions. The new PBP from all strains that have been examined has an identical mobility on SDS gel electrophoresis and is the only PBP still present in an uncomplexed state with beta-lactams (and therefore the only functional PBP when these strains are grown in media containing concentrations of beta-lactam antibiotics sufficient to kill sensitive strains.
耐甲氧西林金黄色葡萄球菌临床分离株对β-内酰胺类抗生素具有内在抗性，因为其耐药机制与β-内酰胺酶的存在无关。我们已经证明，这些菌株中存在一种新的高分子量青霉素结合蛋白（PBP），它对β-内酰胺类抗生素的亲和力较低，并且其含量受生长条件调节。所有已检测菌株的新PBP在SDS凝胶电泳上具有相同的迁移率，并且是唯一仍以未与β-内酰胺类抗生素结合的状态存在的PBP（因此当这些菌株在含有足以杀死敏感菌株的β-内酰胺类抗生素浓度的培养基中生长时，它是唯一具有功能的PBP）。
