Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education and Xinjiang Uyghur Autonomous Region, School of Chemical Engineering and Technology, Xinjiang University, Urumqi 830017, China.
State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources College of Chemistry, Xinjiang University, Urumqi, Xinjiang 830017, China.
Bioelectrochemistry. 2024 Dec;160:108758. doi: 10.1016/j.bioelechem.2024.108758. Epub 2024 Jun 12.
For the electrodeposition, the conductivity and lattice structure of substrate is important to the morphology and lattice of the deposited material. In this study, gold-platinum (AuPt) nanopartical was deposited on nickel foam (NF) based on the lattice induced orientation of the Ni substrate, and the obtained AuPt/NF was applied to construct electrochemical impedimetric immunosensor for procalcitonin (PCT) detection. As a new immunosensor matrix, NF with higher electrical conductance, flexibility and specific surface area, which can improve the plasticity, sensitivity and universality of the immunoelectrode. Due to the lattice matching between Au and Ni, ultrathin AuPt nanolayer with good biocompatibility and large surface area can be modified on the NF surface, which can bind more biomolecules and amplifies the change of impedance signal. Based on the synergistic effect between AuPt and NF, PCT detection based on this electrochemical impedimetric immunosensor with a wide linear range (0.2 pg mL to 20 ng mL) and low detection limit (0.11 pg mL). In addition, this impedimetric immunosensor exhibits high recovery in the PCT detection of serum samples. This work provides a new thought and method for the construction of electrochemical immunosensor.
对于电沉积而言，基底的导电性和晶格结构对于沉积材料的形态和晶格结构非常重要。在本研究中，基于 Ni 基底的晶格诱导取向，在泡沫镍（NF）上沉积了金铂（AuPt）纳米颗粒，并将得到的 AuPt/NF 用于构建用于降钙素原（PCT）检测的电化学阻抗免疫传感器。作为一种新的免疫传感器基质，NF 具有更高的电导率、柔韧性和比表面积，这可以提高免疫电极的可塑性、灵敏度和通用性。由于 Au 和 Ni 之间的晶格匹配，可以在 NF 表面修饰具有良好生物相容性和大表面积的超薄 AuPt 纳米层，这可以结合更多的生物分子并放大阻抗信号的变化。基于 AuPt 和 NF 的协同作用，基于这种具有较宽线性范围（0.2 pg mL 至 20 ng mL）和较低检测限（0.11 pg mL）的电化学阻抗免疫传感器进行了 PCT 检测。此外，这种阻抗免疫传感器在血清样品的 PCT 检测中表现出较高的回收率。这项工作为电化学免疫传感器的构建提供了新的思路和方法。
