多粘类芽孢杆菌 188 的分离与基因组特征分析，一种有潜力的真菌生防菌。

Isolation and genome characterization of Paenibacillus polymyxa 188, a potential biocontrol agent against fungi.

机构信息

Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung City, 804, Taiwan.

Eastern Marine Biology Research Center, Taitung City, 950, Taiwan.

出版信息

J Appl Microbiol. 2024 Apr 1;135(4). doi: 10.1093/jambio/lxae075.

DOI:10.1093/jambio/lxae075

PMID:38509027

Abstract

AIMS

In this work, we aimed to isolate marine bacteria that produce metabolites with antifungal properties.

METHODS AND RESULTS

Paenibacillus polymyxa 188 was isolated from a marine sediment sample, and it showed excellent antifungal activity against many fungi pathogenic to plants (Fusarium tricinctum, Pestalotiopsis clavispora, Fusarium oxysporum, F. oxysporum f. sp. Cubense (Foc), Curvularia plantarum, and Talaromyces pinophilus) and to humans (Aspergillus terreus, Penicillium oxalicum, and Microsphaeropsis arundinis). The antifungal compounds produced by P. polymyxa 188 were extracted and analyzed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The complete genome sequence and biosynthetic gene clusters of P. polymyxa 188 were characterized and compared with those of other strains. A total of 238 carbohydrate-active enzymes (CAZymes) were identified in P. polymyxa 188. Two antibiotic gene clusters, fusaricidin and tridecaptin, exist in P. polymyxa 188, which is different from other strains that typically have multiple antibiotic gene clusters.

CONCLUSIONS

Paenibacilluspolymyxa 188 was identified with numerous biosynthetic gene clusters, and its antifungal ability against pathogenic fungi was verified.

摘要

目的

在这项工作中，我们旨在分离产生具有抗真菌特性代谢物的海洋细菌。

方法和结果

从海洋沉积物样本中分离出多粘类芽孢杆菌 188，它对许多植物病原菌（三隔镰刀菌、拟盘多毛孢、尖孢镰刀菌、尖孢镰刀菌古巴专化型（Foc）、旋孢腔菌和塔宾曲霉）和人类病原菌（土曲霉、草酸青霉和小尾孢）具有优异的抗真菌活性。多粘类芽孢杆菌 188 产生的抗真菌化合物使用基质辅助激光解吸电离飞行时间质谱法进行提取和分析。对多粘类芽孢杆菌 188 的全基因组序列和生物合成基因簇进行了表征，并与其他菌株进行了比较。在多粘类芽孢杆菌 188 中鉴定出 238 种碳水化合物活性酶（CAZymes）。多粘类芽孢杆菌 188 中存在 fusaricidin 和 tridecaptin 两种抗生素基因簇，这与通常具有多个抗生素基因簇的其他菌株不同。