Bakr Noura Mohammed, Balbola Gihan A, Gawad Mohamed Nora Abdel, Ahmed Nehad A, Sapri Ahmed Mohammed, Mously Eihab A, Felemban Doaa, Elsayed Shadia A, Hassan Sandy
Department of Oral and Dental Biology, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Department of Oral and Dental Pathology, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Heliyon. 2024 Mar 7;10(6):e27495. doi: 10.1016/j.heliyon.2024.e27495. eCollection 2024 Mar 30.
Radiation therapy produces reactive oxygen species, which have been linked to various degenerative conditions in periodontal attachment. This study aimed to assess the beneficial effects of aqueous leaf extract on the periodontium of albino rats exposed to fractionated gamma radiation.
This experimental study involved 24 adult male albino rats divided into three groups: Group M received leaf extract (300 mg/kg) intraperitoneally for 14 days; Group R received 20 Gy fractionated gamma irradiation; and Group MR received the same regimen as Group M and then fractionated gamma irradiation dose as Group R. On the first and seventh days post-radiation, bone, cementum, and periodontal ligament samples were histologically and histomorphometrically examined.
The periodontal ligament, alveolar bone, and cementum showed structural damage in Group R. A relative persistence of normal periodontal tissue structures was seen in Group MR, showing less disruption of the periodontal ligament and greater trabecular bone thickness than Group R. The histomorphometric analysis showed that the mean periodontal ligament width was highest in Group R7 (245.20 μm) and lowest in Group M7 (54.55 μm). In addition, the mean cementum width was highest in Group R1 (88.99 μm) and lowest in Group M1R1 (17.87 μm) and differed significantly between groups.
Within the limitations of this study, leaf aqueous extract showed the potential to reduce the adverse effects of radiation, control inflammation, and support tissue healing in a rat model.
放射治疗会产生活性氧，这与牙周附着的各种退行性病变有关。本研究旨在评估水提叶提取物对白化大鼠经分次伽马辐射后的牙周组织的有益作用。
本实验研究涉及24只成年雄性白化大鼠，分为三组：M组腹腔注射叶提取物（300mg/kg），持续14天；R组接受20Gy分次伽马辐射；MR组接受与M组相同的方案，然后接受与R组相同的分次伽马辐射剂量。在辐射后的第一天和第七天，对骨、牙骨质和牙周韧带样本进行组织学和组织形态计量学检查。
R组的牙周韧带、牙槽骨和牙骨质出现结构损伤。MR组可见正常牙周组织结构相对持续存在，与R组相比，牙周韧带破坏较少，小梁骨厚度更大。组织形态计量学分析显示，牙周韧带平均宽度在R7组最高（245.20μm），在M7组最低（54.55μm）。此外，牙骨质平均宽度在R1组最高（88.99μm），在M1R1组最低（17.87μm），组间差异显著。
在本研究的局限性内，叶水提取物在大鼠模型中显示出减轻辐射不良反应、控制炎症和支持组织愈合的潜力。
