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组学数据分析揭示了细胞氨基酸组成的系统水平限制。

Omics data analysis reveals the system-level constraint on cellular amino acid composition.

作者信息

Huang Yuanyuan, Mao Zhitao, Zhang Yue, Zhao Jianxiao, Luan Xiaodi, Wu Ke, Yun Lili, Yu Jing, Shi Zhenkun, Liao Xiaoping, Ma Hongwu

机构信息

College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.

Biodesign Center, Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

出版信息

Synth Syst Biotechnol. 2024 Mar 5;9(2):304-311. doi: 10.1016/j.synbio.2024.03.001. eCollection 2024 Jun.

DOI:10.1016/j.synbio.2024.03.001
PMID:38510205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10951587/
Abstract

Proteins play a pivotal role in coordinating the functions of organisms, essentially governing their traits, as the dynamic arrangement of diverse amino acids leads to a multitude of folded configurations within peptide chains. Despite dynamic changes in amino acid composition of an individual protein (referred to as AAP) and great variance in protein expression levels under different conditions, our study, utilizing transcriptomics data from four model organisms uncovers surprising stability in the overall amino acid composition of the total cellular proteins (referred to as AACell). Although this value may vary between different species, we observed no significant differences among distinct strains of the same species. This indicates that organisms enforce system-level constraints to maintain a consistent AACell, even amid fluctuations in AAP and protein expression. Further exploration of this phenomenon promises insights into the intricate mechanisms orchestrating cellular protein expression and adaptation to varying environmental challenges.

摘要

蛋白质在协调生物体功能方面发挥着关键作用，实际上决定着生物体的特征，因为不同氨基酸的动态排列导致肽链内形成多种折叠构型。尽管单个蛋白质的氨基酸组成（称为AAP）存在动态变化，且在不同条件下蛋白质表达水平差异很大，但我们利用四种模式生物的转录组学数据进行的研究发现，总细胞蛋白质的整体氨基酸组成（称为AACell）具有惊人的稳定性。尽管该值在不同物种之间可能有所不同，但我们观察到同一物种的不同菌株之间没有显著差异。这表明，即使在AAP和蛋白质表达波动的情况下，生物体也会实施系统层面的限制，以维持一致的AACell。对这一现象的进一步探索有望深入了解协调细胞蛋白质表达和适应各种环境挑战的复杂机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/c1d27ca10e44/mmcfigs6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/577293cbc935/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/573cbbe72d56/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/cca7fe4d96e3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/e6f8f3f5f4f9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/550226a51898/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/c8ba5c5bd884/mmcfigs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/1ec5618ed78c/mmcfigs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/3893bf17092c/mmcfigs4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/0b0a56c86cad/mmcfigs5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/c1d27ca10e44/mmcfigs6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/577293cbc935/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/573cbbe72d56/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/cca7fe4d96e3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/e6f8f3f5f4f9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/550226a51898/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/c8ba5c5bd884/mmcfigs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/1ec5618ed78c/mmcfigs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/3893bf17092c/mmcfigs4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/0b0a56c86cad/mmcfigs5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3d7/10951587/c1d27ca10e44/mmcfigs6.jpg

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