University of Missouri-Kansas City School of Medicine, Kansas City, Missouri, USA.
Division of Pediatric Clinical Pharmacology and Therapeutic Innovation and Section of Allergy/Asthma/Immunology, Children's Mercy Hospital, Kansas City, Missouri, USA.
Clin Transl Sci. 2024 Mar;17(3):e13777. doi: 10.1111/cts.13777.
The phenotypical manifestations of asthma among children are diverse and exhibit varying responses to therapeutic interventions. There is a need to develop objective biomarkers to improve the characterization of allergic and inflammatory responses relevant to asthma to predict therapeutic treatment responses. We have previously investigated histamine iontophoresis with laser Doppler flowmetry (HILD) as a potential surrogate biomarker that characterizes histamine response and may be utilized to guide the treatment of allergic and inflammatory disease. We have identified intra-individual variability of HILD response type among children and adults with asthma and that HILD response type varied in association with racial classification. As laser Doppler flowimetry may be impacted by skin color, we aimed to further validate the HILD method by determining if skin color or tone is associated with observed HILD response type differences. We conducted an observational study utilizing quantification of skin color and tone obtained from photographs of the skin among participants during HILD assessments via the RGB color model. We compared RGB values across racial, ethnic, and HILD response type via the Kruskal-Wallis test and calculated Kendall rank correlation coefficient to evaluate the relationship between RGB composite scores and HILD pharmacodynamic measures. We observed that RGB scores differed among racial groups and histamine response phenotypes (p < 0.05). However, there was a lack of correlation between the RGB composite score and HILD pharmacodynamic measures (r values 0.1, p > 0.05). These findings suggest that skin color may not impact HILD response variations, necessitating further research to understand previously observed differences across identified racial groups.
儿童哮喘的表型表现多种多样，对治疗干预的反应也各不相同。因此，有必要开发客观的生物标志物来改善与哮喘相关的过敏和炎症反应的特征描述，以预测治疗反应。我们之前已经研究了组胺离子电泳与激光多普勒血流测量（HILD）作为一种潜在的替代生物标志物，它可以描述组胺反应，并可用于指导过敏和炎症性疾病的治疗。我们已经确定了哮喘儿童和成人中 HILD 反应类型的个体内可变性，并且 HILD 反应类型与种族分类有关。由于激光多普勒血流测量可能会受到皮肤颜色的影响，我们旨在通过确定皮肤颜色或色调是否与观察到的 HILD 反应类型差异有关，进一步验证 HILD 方法。我们通过利用 RGB 颜色模型在 HILD 评估期间从参与者的皮肤照片中获得的皮肤颜色和色调的量化，进行了一项观察性研究。我们通过 Kruskal-Wallis 检验比较了不同种族、民族和 HILD 反应类型之间的 RGB 值，并计算 Kendall 秩相关系数来评估 RGB 综合评分与 HILD 药效学测量之间的关系。我们观察到 RGB 评分在不同种族群体和组胺反应表型之间存在差异（p<0.05）。然而，RGB 综合评分与 HILD 药效学测量之间没有相关性（r 值为 0.1，p>0.05）。这些发现表明皮肤颜色可能不会影响 HILD 反应的变化，需要进一步研究以了解之前在确定的种族群体中观察到的差异。
