Department of Veterinary Gynaecology and Obstetrics, Bihar Veterinary College, Bihar Animal Sciences University, Patna, Bihar 800014, India.
Department of Veterinary Biochemistry, Bihar Veterinary College, Bihar Animal Sciences University, Patna, Bihar 800014, India.
Pol J Vet Sci. 2024 Mar 20;27(1):43-52. doi: 10.24425/pjvs.2024.149332.
Cystic endometrial hyperplasia-pyometra complex (CEH-P) is a common disease in sexually mature bitches. Disease progression leads to oxidative stress, resulting in the depletion of uterine antioxidants and lipid peroxidation of associated cells, which further aggravates the condition. The concentration of antioxidant enzymes, the level of lipid peroxidation within the uterine tissue, and its reflection in the serum and urine need to be elucidated. The aim of this study was to analyze the concentration of antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), and the lipid peroxidation marker malonaldehyde (MDA) in three types of samples, i.e., serum, urine, and uterine tissue. For this purpose, 58 pyometra-affected and 44 healthy bitches were included in the present study. All animals underwent ovariohysterectomy (OVH). Our data indicated highly significant difference (p<0.01) in the antioxidant concentrations of uterine, serum and urine samples. Furthermore, there was a highly significant (p<0.01) difference in the serum levels of ferric reducing antioxidant power (FRAP) and free radical scavenging activity (FRSA) indicated poor capacity to overcome oxidative stress in the CEH-Pyometra condition. We showed that CEH-P induces oxidative stress, which further depletes the antioxidant enzyme reserves in the uterus. Thus, the weak antioxidant defence predisposes to uterine damage and disease progression. The simultaneous depletion of antioxidants and an increase in lipid peroxidation in the serum and urine may also act as early indicators of uterine pathology.
囊性子宫内膜增生-子宫积脓综合征（CEH-P）是性成熟犬的一种常见疾病。疾病的进展导致氧化应激，导致子宫抗氧化剂耗竭和相关细胞的脂质过氧化，从而进一步加重病情。需要阐明抗氧化酶的浓度、子宫组织内的脂质过氧化水平及其在血清和尿液中的反映。本研究的目的是分析三种类型的样本（血清、尿液和子宫组织）中的抗氧化剂如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）和脂质过氧化标志物丙二醛（MDA）的浓度。为此，本研究纳入了 58 例子宫积脓犬和 44 例健康犬。所有动物均接受了卵巢子宫切除术（OVH）。我们的数据表明，子宫、血清和尿液样本中的抗氧化剂浓度存在显著差异（p<0.01）。此外，CEH-Pyometra 状态下血清铁还原抗氧化能力（FRAP）和自由基清除活性（FRSA）水平存在显著差异（p<0.01），表明抗氧化能力差，无法克服氧化应激。我们表明，CEH-P 会诱导氧化应激，进一步耗尽子宫中的抗氧化酶储备。因此，抗氧化防御能力较弱会导致子宫损伤和疾病进展。同时在血清和尿液中耗竭抗氧化剂和增加脂质过氧化也可能作为子宫病理学的早期指标。
