Electron microscopical-immunocytochemical evidence of ecdysteroids in the prothoracic gland of Galleria mellonella.

Fixation of prothoracic glands of Galleria mellonella with a solution containing saponin permits immunocytochemical staining of the entire gland. By this means ecdysteroids were demonstrated electron microscopically to be present in the hyaloplasm and microtubules.