School of Chemistry and Material Science, East China University of Technology, Nanchang, China.
School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, China.
Luminescence. 2024 Mar;39(3):e4713. doi: 10.1002/bio.4713.
As large numbers of people are suffering from gout, an accurate, rapid, and sensitive method for the detection of gout biomarker, uric acid, is important for its effective control, diagnosis, and therapy. Although colorimetric detection methods based on uricase have been considered, they still have limitations as they produce toxic HO and are expensive and not stable. Here, a novel uricase-free colorimetric method was developed for the sensitive and selective detection of uric acid based on the light-induced oxidase-mimicking activity of a new photosensitized covalent organic framework (COF) (2,4,6-trimethylpyridine-3,5-dicarbonitrile-4-[2-(4-formylphenyl)ethynyl]benzaldehyde COF [DCTP-EDA COF]). DCTP-EDA COF has a strong ability to harvest visible light, and it could catalyze the oxidation of 1,4-dioxane, 3,3',5,5'-tetramethylbenzidine under visible light irradiation to produce obvious color changes. With the addition of uric acid, however, the significant inhibition of the oxidase-mimicking activity of DCTP-EDA COF remarkably faded the color, and thus uric acid could be colorimetrically detected in the range of 2.0-150 μM with a limit of detection of 0.62 μM (3σ/K). Moreover, the present colorimetric method exhibited high selectivity; uric acid level in serum samples was successfully determined, and the recoveries ranged from 96.5% to 105.64%, suggesting the high accuracy of the present colorimetric method, which demonstrates great promise in clinical analysis.
由于大量人群患有痛风，因此准确、快速、灵敏地检测痛风生物标志物尿酸对于其有效控制、诊断和治疗非常重要。虽然已经考虑了基于尿酸酶的比色检测方法，但由于它们会产生有毒的 HO，而且昂贵且不稳定，因此它们仍然存在局限性。在这里，我们基于一种新的光敏共价有机框架（COF）（2,4,6-三甲基吡啶-3,5-二氰基-4-[2-（4-甲酰基苯基）乙炔基]苯甲醛 COF [DCTP-EDA COF]）开发了一种新型的尿酸酶免费比色法，用于灵敏和选择性地检测尿酸。DCTP-EDA COF 具有很强的可见光收集能力，并且可以在可见光照射下催化 1,4-二恶烷和 3,3',5,5'-四甲基联苯胺的氧化，从而产生明显的颜色变化。然而，随着尿酸的加入，DCTP-EDA COF 的氧化酶模拟活性受到显著抑制，导致颜色明显褪色，从而可以在 2.0-150 μM 的范围内比色检测尿酸，检测限为 0.62 μM（3σ/K）。此外，该比色法具有高选择性；成功测定了血清样品中的尿酸水平，回收率范围为 96.5%至 105.64%，表明该比色法具有很高的准确性，在临床分析中具有很大的应用前景。
