Nakata Y, Yamashita J, Kishi T, Kataoka M, Ejiri T, Ohnoshi T, Kimura I
Cancer Immunol Immunother. 1985;20(1):43-6. doi: 10.1007/BF00199772.
In vivo animal studies support the concept that monocytes and macrophages are important in the immune surveillance of oncogenesis and that in vitro activated murine macrophages are cytocidal for tumour cells. In this study, the tumour cell cytotoxic activity of human peripheral blood monocytes was examined by measuring the inhibition of 3H-thymidine uptake in the human cancer cell line, established in our laboratory from human squamous cell lung cancer. The monocytes from 8 of the 31 lung cancer patients (26%) showed a percentage growth inhibition of less than 69.8%, which exceeded the 95% confidence limits of the percentage growth inhibition observed with healthy control monocytes. On the other hand, among the 16 sarcoidosis and the 8 tuberculosis cases no value was below 69.8%. However, there was no significant difference between the growth inhibition and the clinical stages or histological type. When OK-432, a Streptococcal agent, was administered in vivo to patients with lung cancer, an elevation of the growth inhibition was observed in 7 out of 8 patients. It was confirmed that the tumour cell cytostatic activity of the monocyte is suppressed in patients with lung cancer, and these monocyte deficits hinder the inhibition of tumour growth and metastasis.
单核细胞和巨噬细胞在肿瘤发生的免疫监视中起重要作用，并且体外活化的小鼠巨噬细胞对肿瘤细胞具有细胞毒性。在本研究中，通过测量对在我们实验室中从人肺鳞状细胞癌建立的人癌细胞系中3H-胸腺嘧啶核苷摄取的抑制作用，检测了人外周血单核细胞的肿瘤细胞细胞毒性活性。31例肺癌患者中有8例（26%）的单核细胞显示生长抑制百分比低于69.8%，这超过了健康对照单核细胞观察到的生长抑制百分比的95%置信限。另一方面，在16例结节病患者和8例结核病患者中，没有数值低于69.8%。然而，生长抑制与临床分期或组织学类型之间没有显著差异。当将链球菌制剂OK-432体内给予肺癌患者时，8例患者中有7例观察到生长抑制升高。证实肺癌患者单核细胞的肿瘤细胞抑制活性受到抑制，并且这些单核细胞缺陷阻碍了肿瘤生长和转移的抑制。
