Yanagawa E, Uchida A, Kokoschka E M, Micksche M
Cancer Immunol Immunother. 1984;16(3):131-6. doi: 10.1007/BF00205418.
Lymphocytes and monocytes from the peripheral blood of 30 patients with malignant melanoma were tested for natural cytotoxicity against K562 cells in a 3-h 51Cr-release assay, and the effects of OK432 (a streptococcal preparation) on the cytotoxicity were examined. The lymphocyte cytotoxicity of melanoma patients was similar to that of normal donors and control patients with benign skin disease. Furthermore, the lymphocyte cytotoxicity of melanoma patients was not correlated to the stage of the disease. Similarly, lysis of K562 cells by monocytes isolated by adherence to autologous serum-coated plastic dishes in melanoma patients was comparable to that of controls and not associated with the stage of the disease. Positive monocyte reactions were recorded in 10 of 30 (33%) melanoma patients, seven of 21 (33%) normal donors and three of 10 (30%) control patients. There was no correlation between lymphocyte cytotoxicity and monocyte cytotoxicity. Overnight treatment of monocytes and lymphocytes with OK432 resulted in an increase in cytotoxicity. Significant augmentation of cytotoxicity by OK432 was observed in 28% of the monocyte samples and 86% of the lymphocyte samples, while partially purified human interferon augmented cytotoxicity in 63% of the monocyte samples and all the lymphocyte samples. These results suggest that neither lymphocyte nor monocyte cytotoxicities are depressed in melanoma patients as compared with normal donors and patients with benign disease and that OK432 has a stronger stimulatory effect on lymphocytes than on monocytes.
在一项3小时的51Cr释放试验中，检测了30例恶性黑色素瘤患者外周血中的淋巴细胞和单核细胞对K562细胞的自然细胞毒性，并研究了OK432（一种链球菌制剂）对细胞毒性的影响。黑色素瘤患者的淋巴细胞细胞毒性与正常供体及良性皮肤疾病对照患者相似。此外，黑色素瘤患者的淋巴细胞细胞毒性与疾病分期无关。同样，通过贴壁于自体血清包被的塑料培养皿分离出的黑色素瘤患者单核细胞对K562细胞的裂解作用与对照组相当，且与疾病分期无关。30例黑色素瘤患者中有10例（33%）、21例正常供体中有7例（33%）以及10例对照患者中有3例（30%）出现单核细胞阳性反应。淋巴细胞细胞毒性与单核细胞细胞毒性之间无相关性。用OK432对单核细胞和淋巴细胞进行过夜处理后，细胞毒性增加。在28%的单核细胞样本和86%的淋巴细胞样本中观察到OK432显著增强了细胞毒性，而部分纯化的人干扰素在63%的单核细胞样本和所有淋巴细胞样本中增强了细胞毒性。这些结果表明，与正常供体和良性疾病患者相比，黑色素瘤患者的淋巴细胞和单核细胞细胞毒性均未降低，且OK432对淋巴细胞的刺激作用比对单核细胞更强。
