Mantovani A, Polentarutti N, Peri G, Shavit Z B, Vecchi A, Bolis G, Mangioni C
J Natl Cancer Inst. 1980 Jun;64(6):1307-15. doi: 10.1093/jnci/64.6.1307.
Mononuclear phagocytes were isolated from the peripheral blood (PB) and ascites tumors of 35 patients with epithelial ovarian tumors. After 48 hours of incubation with the TU5 tumor, tumor-associated macrophages (TAM) and PB monocytes from cancer patients showed lower cytolytic activity than did control cells, but by 72 hours there was little difference between control and ovarian cancer effector cells. Primary ovarian carcinoma cultures were heterogeneous in their susceptibility to macrophage cytotoxicity. Tumor cells from 7 patients were significantly lysed by monocytes and macrophages, whereas four ovarian cancer cell preparations were resistant to cytotoxicity. A "feeding" effect of mononuclear phagocytes on non-lysable tumor cells was detected in terms of both lower [3H]thymidine-release values in the cytolysis assay and increased proliferation in cytostasis assays. Thus patients with ovarian carcinomatous ascites PB monocytes and TAM had impaired cytotoxicity against a tumor cell line, and primary ovarian carcinoma cultures were heterogeneous in their interaction with mononuclear phagocytes.
从35例上皮性卵巢肿瘤患者的外周血（PB）和腹水肿瘤中分离出单核吞噬细胞。在用TU5肿瘤孵育48小时后，癌症患者的肿瘤相关巨噬细胞（TAM）和PB单核细胞的细胞溶解活性低于对照细胞，但到72小时时，对照细胞和卵巢癌效应细胞之间几乎没有差异。原发性卵巢癌培养物对巨噬细胞细胞毒性的敏感性存在异质性。7例患者的肿瘤细胞被单核细胞和巨噬细胞显著裂解，而4种卵巢癌细胞制剂对细胞毒性具有抗性。在细胞溶解试验中较低的[3H]胸苷释放值和细胞增殖试验中增加的增殖方面，检测到单核吞噬细胞对不可裂解肿瘤细胞的“滋养”作用。因此，患有卵巢癌腹水的PB单核细胞和TAM对肿瘤细胞系的细胞毒性受损，并且原发性卵巢癌培养物在与单核吞噬细胞的相互作用中存在异质性。
