Bagasra O, Berman W, Hashemi S, Howeedy A, Kushner H
Cancer Immunol Immunother. 1985;20(1):55-60. doi: 10.1007/BF00199774.
The formation of tumor stem cell colonies in vitro has been studied by comparing the growth of three mouse teratocarcinoma derived cell lines and one human teratocarcinoma derived cell line in semi-solid media containing either agar or agarose. We show that agaroses should be used in higher concentrations than agar to obtain comparable results. The maximum number of colonies were obtained in agarose over a broader range of concentrations (1%-4% for SeaPrep and 0.5%-2% for SeaPlaque agarose) than in agar, which allowed anchorage-independent growth of tumor cells only over a narrow concentration range (0.3%-0.5%). Overall, the preparation of media containing agarose was less cumbersome than preparation of agar-containing media, primarily because agaroses gelled more slowly and remained liquid in the physiological temperature range. Furthermore, the transfer of colonies from semi-solid media containing agarose to solid surface tissue culture dishes was much more efficient than the transfer of colonies from agar. The stock solutions of SeaPrep agarose could be kept ready for use for extended periods of time. All these features show that the low melting point agarose has considerable advantages over agar for preparation of semi-solid media for anchorage-independent tumor cell growth.
通过比较三种小鼠畸胎瘤衍生细胞系和一种人畸胎瘤衍生细胞系在含有琼脂或琼脂糖的半固体培养基中的生长情况，对肿瘤干细胞集落的体外形成进行了研究。我们发现，为获得可比结果，琼脂糖的使用浓度应高于琼脂。与琼脂相比，在更宽的浓度范围内（SeaPrep琼脂糖为1%-4%，SeaPlaque琼脂糖为0.5%-2%），琼脂糖能获得更多的集落，而琼脂仅在狭窄的浓度范围（0.3%-0.5%）内允许肿瘤细胞进行非锚定依赖性生长。总体而言，含琼脂糖培养基的制备比含琼脂培养基的制备更简便，主要是因为琼脂糖凝胶化更慢，在生理温度范围内保持液态。此外，将集落从含琼脂糖的半固体培养基转移到固体表面组织培养皿比从琼脂转移更高效。SeaPrep琼脂糖储备液可长时间备用。所有这些特性表明，低熔点琼脂糖在制备用于非锚定依赖性肿瘤细胞生长的半固体培养基方面比琼脂具有相当大的优势。
