Oosterhuis J W, Bagasra O, Kushner H, Fox N, Damjanov I
Br J Cancer. 1983 Mar;47(3):407-11. doi: 10.1038/bjc.1983.61.
Murine embryonal carcinoma cells, the pluripotent stem cells of teratocarcinoma were injected simultaneously into caudal and cranial sites on the back of syngeneic recipients in order to determine whether regional anatomical differences affect their take and growth rate and differentiation. The overall tumour take rate was higher in caudal than cranial sites, but the initial weight of tumours was higher in the cranial than caudal sites. Tumours developing in the two anatomical sites grew at the same rate with a linear increase in volume. At the end of the 4-week experimental period the differences in the size of anterior and posterior tumours were negligible and no histological differences were noted between the two groups. Our data indicate that regional factors significantly affect the take rate and the initial growth of this murine teratocarcinoma, i.e. the establishment of solid tumours from injected stem cells. The growth rate of established tumours was not affected by regional factors.
为了确定区域解剖差异是否会影响其植入率、生长速度和分化情况，将小鼠胚胎癌细胞（一种畸胎癌的多能干细胞）同时注射到同基因受体背部的尾部和头部位置。总体而言，尾部的肿瘤植入率高于头部，但肿瘤的初始重量在头部高于尾部。在两个解剖部位生长的肿瘤生长速度相同，体积呈线性增加。在为期4周的实验期结束时，前后肿瘤大小的差异可以忽略不计，两组之间未观察到组织学差异。我们的数据表明，区域因素显著影响这种小鼠畸胎癌的植入率和初始生长，即从注射的干细胞形成实体瘤。已形成肿瘤的生长速度不受区域因素的影响。
