Sakurai A, Satomi N, Haranaka K
Cancer Immunol Immunother. 1985;20(1):6-10. doi: 10.1007/BF00199766.
The relationship between tumour necrosis factor (TNF) and macrophages or macrophage-like cell line, especially the lysosomal enzymes was investigated. The serum lysosomal enzymes and LDH activities were increased in proportion to the TNF production even in different strains of mice. Lysosomal enzymes and TNF activity were released into the supernatant of the culture medium of macrophage-enriched peritoneal exudate cells (PEC) or spleen cells derived from Propionibacterium acnes-primed mice after addition of lipopolysaccharide (LPS). After passage through a Sephadex G-10 column, TNF activity could not be detected in the supernatant of these spleen cells after addition of LPS. Also TNF activity could not be detected in the supernatant following destruction of PEC. These results suggest that TNF producibility is strongly related to the degree of activation of macrophages, especially the lysosomal enzymes. The murine macrophage-like cell line, J774, also released TNF activity and lysosomal enzymes after addition of LPS.
研究了肿瘤坏死因子（TNF）与巨噬细胞或巨噬细胞样细胞系之间的关系，尤其是与溶酶体酶的关系。即使在不同品系的小鼠中，血清溶酶体酶和乳酸脱氢酶（LDH）活性也与TNF的产生成比例增加。在添加脂多糖（LPS）后，溶酶体酶和TNF活性从痤疮丙酸杆菌致敏小鼠的富含巨噬细胞的腹腔渗出细胞（PEC）或脾细胞的培养基上清液中释放出来。通过葡聚糖G-10柱后，添加LPS后这些脾细胞的上清液中未检测到TNF活性。PEC破坏后上清液中也未检测到TNF活性。这些结果表明，TNF的产生能力与巨噬细胞的激活程度密切相关，尤其是与溶酶体酶有关。小鼠巨噬细胞样细胞系J774在添加LPS后也释放出TNF活性和溶酶体酶。
