Haranaka K, Satomi N, Sakurai A
Br J Cancer. 1984 Oct;50(4):471-8. doi: 10.1038/bjc.1984.203.
Large differences in tumour necrosis factor (TNF) productive ability 05337257erved among various strains of mice. DDY, CD-1, ICR and DBA/2 mice could produce a high titre of TNF activity, whereas Balb/c, C3H/HeJms and A/J mice produced a low titre of TNF activity. Administration of 200 micrograms/mouse of LPS to some strains of mice, i.e. DDD and C57B1/6J resulted in good production of TNF. ICR nu/nu mice produced the highest TNF activity among the nude mice. Balb/c nu/nu and DDD nu/nu mice exhibited very low titres of TNF activity. Nude mice required a rather higher dose of the priming agent, Propionibacterium acnes, than heterozygote littermates. Although it is commonly accepted that dual stimulation is necessary for TNF production, TNF activity was detected without the priming agent in SD rats and Golden hamsters by single injection of LPS. In these animals, much higher TNF production was observed after Propionibacterium acnes treatment than after a single injection of LPS. Large differences in TNF productive ability also existed among strains of rats. Although all animals receiving priming agents revealed hyperplasia of reticuloendothelial system, the sensitivity of the animals to LPS is considered to be the most important factor in their TNF productive ability.
在不同品系的小鼠中观察到肿瘤坏死因子（TNF）产生能力存在很大差异。DDY、CD-1、ICR和DBA/2小鼠能够产生高滴度的TNF活性，而Balb/c、C3H/HeJms和A/J小鼠产生的TNF活性滴度较低。给某些品系的小鼠，即DDD和C57B1/6J小鼠每只注射200微克脂多糖（LPS），可促使其产生大量TNF。ICR裸鼠在裸鼠中产生的TNF活性最高。Balb/c裸鼠和DDD裸鼠的TNF活性滴度非常低。与杂合子同窝小鼠相比，裸鼠需要更高剂量的启动剂痤疮丙酸杆菌。尽管普遍认为双重刺激是产生TNF所必需的，但在SD大鼠和金黄仓鼠中，通过单次注射LPS，在没有启动剂的情况下也检测到了TNF活性。在这些动物中，痤疮丙酸杆菌处理后观察到的TNF产生量比单次注射LPS后高得多。大鼠品系之间在TNF产生能力上也存在很大差异。尽管所有接受启动剂的动物都出现了网状内皮系统增生，但动物对LPS的敏感性被认为是其TNF产生能力的最重要因素。
