• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人类β-珠蛋白和γ-珠蛋白前体mRNA的两个间隔序列在体外以优先的时间顺序被切除。

The two intervening sequences of human beta- and gamma-globin pre-mRNAs are excised in a preferred temporal order in vitro.

作者信息

Lang K M, van Santen V L, Spritz R A

出版信息

EMBO J. 1985 Aug;4(8):1991-6. doi: 10.1002/j.1460-2075.1985.tb03882.x.

DOI:10.1002/j.1460-2075.1985.tb03882.x
PMID:3851741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554452/
Abstract

Splicing of complete synthetic human beta- and gamma-globin mRNA precursors (pre-mRNAs) follows a preferred pathway in vitro. In almost all molecules, excision of the first intervening sequence (IVS) is completed before excision of the second IVS initiates. However, in a very small fraction of molecules the order of IVS excision is reversed.

摘要

完整的合成人β-和γ-珠蛋白mRNA前体（前体mRNA）的剪接在体外遵循一种优先途径。在几乎所有分子中，第一个内含子序列（IVS）的切除在第二个IVS切除开始之前就已完成。然而，在极少数分子中，IVS切除的顺序是相反的。

相似文献

1
The two intervening sequences of human beta- and gamma-globin pre-mRNAs are excised in a preferred temporal order in vitro.人类β-珠蛋白和γ-珠蛋白前体mRNA的两个间隔序列在体外以优先的时间顺序被切除。
EMBO J. 1985 Aug;4(8):1991-6. doi: 10.1002/j.1460-2075.1985.tb03882.x.
2
Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.正常和突变的人β-珠蛋白前体mRNA在体外能够被准确且高效地剪接。
Cell. 1984 Apr;36(4):993-1005. doi: 10.1016/0092-8674(84)90049-7.
3
A transcription map for the rabbit beta-globin gene.兔β-珠蛋白基因的转录图谱。
Cell. 1981 Feb;23(2):573-84. doi: 10.1016/0092-8674(81)90153-7.
4
Deletion analysis of the human gamma-globin IVS2: sequence requirements for RNA splicing.人类γ-珠蛋白IVS2的缺失分析：RNA剪接的序列要求
Ann N Y Acad Sci. 1985;445:10-9. doi: 10.1111/j.1749-6632.1985.tb17170.x.
5
Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.在体外前体mRNA剪接过程中，完整内含子作为一种新型套索结构被切除。
Cell. 1984 Aug;38(1):317-31. doi: 10.1016/0092-8674(84)90553-1.
6
Sequence organization of the beta-globin mRNA precursor.β-珠蛋白信使核糖核酸前体的序列组织
Nucleic Acids Res. 1978 Sep;5(9):3295-301. doi: 10.1093/nar/5.9.3295.
7
In vitro splicing pathways of pre-mRNAs containing multiple intervening sequences?含有多个居间序列的前体mRNA的体外剪接途径？
Mol Cell Biol. 1987 Oct;7(10):3428-37. doi: 10.1128/mcb.7.10.3428-3437.1987.
8
mRNA precursor splicing in vivo: sequence requirements determined by deletion analysis of an intervening sequence.体内信使核糖核酸前体剪接：通过间隔序列缺失分析确定的序列要求
Proc Natl Acad Sci U S A. 1985 May;82(9):2885-9. doi: 10.1073/pnas.82.9.2885.
9
5' cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5' splice region, not by the conserved 5' GU.真核生物前体mRNA剪接中的5'切割位点由整个5'剪接区域决定，而非保守的5'GU。
Cell. 1987 Jul 17;50(2):237-46. doi: 10.1016/0092-8674(87)90219-4.
10
The precursor of mouse beta-globin messenger RNA contains two intervening RNA sequences.小鼠β-珠蛋白信使核糖核酸的前体含有两个居间的核糖核酸序列。
Cell. 1978 Jul;14(3):681-93. doi: 10.1016/0092-8674(78)90251-9.

引用本文的文献

1
Rare autosomal recessive cardiac valvular form of Ehlers-Danlos syndrome results from mutations in the COL1A2 gene that activate the nonsense-mediated RNA decay pathway.罕见的常染色体隐性遗传性埃勒斯-当洛综合征心脏瓣膜型是由COL1A2基因突变引起的，这些突变激活了无义介导的RNA降解途径。
Am J Hum Genet. 2004 May;74(5):917-30. doi: 10.1086/420794. Epub 2004 Apr 9.
2
Redefinition of exon 7 in the COL1A1 gene of type I collagen by an intron 8 splice-donor-site mutation in a form of osteogenesis imperfecta: influence of intron splice order on outcome of splice-site mutation.Ⅰ型胶原COL1A1基因外显子7的重新定义：由成骨不全一种形式中的内含子8剪接供体位点突变所致，内含子剪接顺序对剪接位点突变结果的影响
Am J Hum Genet. 1999 Aug;65(2):336-44. doi: 10.1086/302512.
3

本文引用的文献

1
A transcription map for the rabbit beta-globin gene.兔β-珠蛋白基因的转录图谱。
Cell. 1981 Feb;23(2):573-84. doi: 10.1016/0092-8674(81)90153-7.
2
Processing of high molecular weight ovalbumin and ovomucoid precursor RNAs to messenger RNA.高分子量卵清蛋白和卵类粘蛋白前体RNA加工成信使RNA的过程。
Cell. 1980 Nov;22(1 Pt 1):219-30. doi: 10.1016/0092-8674(80)90170-1.
3
Identification, organization and processing intermediates of the putative precursors of Xenopus vitellogenin messenger RNA.非洲爪蟾卵黄蛋白原信使核糖核酸假定前体的鉴定、组织及加工中间体
Translation of a retained intron in tyrosinase-related protein (TRP) 2 mRNA generates a new cytotoxic T lymphocyte (CTL)-defined and shared human melanoma antigen not expressed in normal cells of the melanocytic lineage.酪氨酸酶相关蛋白（TRP）2信使核糖核酸（mRNA）中保留内含子的翻译产生了一种新的细胞毒性T淋巴细胞（CTL）定义的且为黑素瘤细胞系正常细胞所不表达的共有的人类黑素瘤抗原。
J Exp Med. 1998 Sep 21;188(6):1005-16. doi: 10.1084/jem.188.6.1005.
4
Sequence of the polypyrimidine tract of the 3'-terminal 3' splicing signal can affect intron-dependent pre-mRNA processing in vivo.3'剪接信号3'末端的多嘧啶序列可影响体内内含子依赖性前体mRNA的加工。
Nucleic Acids Res. 1996 May 1;24(9):1765-73. doi: 10.1093/nar/24.9.1765.
5
Polyadenylation site selection cannot occur in vivo after excision of the 3'-terminal intron.3'-末端内含子切除后，聚腺苷酸化位点选择无法在体内发生。
Nucleic Acids Res. 1993 Nov 11;21(22):5256-63. doi: 10.1093/nar/21.22.5256.
6
Two spliceosomes can form simultaneously and independently on synthetic double-intron messenger RNA precursors.两个剪接体可以在合成的双内含子信使核糖核酸前体上同时且独立地形成。
EMBO J. 1987 Jun;6(6):1747-55. doi: 10.1002/j.1460-2075.1987.tb02427.x.
7
In vitro splicing pathways of pre-mRNAs containing multiple intervening sequences?含有多个居间序列的前体mRNA的体外剪接途径？
Mol Cell Biol. 1987 Oct;7(10):3428-37. doi: 10.1128/mcb.7.10.3428-3437.1987.
8
Alternative splicing of SV40 early pre-mRNA in vitro.猿猴病毒40早期前体信使核糖核酸在体外的可变剪接
Nucleic Acids Res. 1986 Dec 22;14(24):9911-26. doi: 10.1093/nar/14.24.9911.
9
Model for alternative RNA processing in human calcitonin gene expression.人降钙素基因表达中替代性RNA加工模型。
Nucleic Acids Res. 1986 Nov 25;14(22):8785-803. doi: 10.1093/nar/14.22.8785.
10
Presence of a polyadenylated RNA fragment encoding the membrane domain for immunoglobulin alpha chain indicates that mRNAs for both secreted and membrane-bound alpha chains can be produced from the same RNA transcript.编码免疫球蛋白α链膜结构域的多聚腺苷酸化RNA片段的存在表明，分泌型和膜结合型α链的mRNA都可以从同一RNA转录本产生。
Nucleic Acids Res. 1986 Aug 11;14(15):6129-44. doi: 10.1093/nar/14.15.6129.
Cell. 1980 Jan;19(1):53-61. doi: 10.1016/0092-8674(80)90387-6.
4
Abnormal RNA processing due to the exon mutation of beta E-globin gene.由于β-珠蛋白基因外显子突变导致的异常RNA加工。
Nature. 1982 Dec 23;300(5894):768-9. doi: 10.1038/300768a0.
5
A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing.β0地中海贫血基因剪接位点的单碱基变化导致异常的RNA剪接。
Cell. 1982 Jul;29(3):903-11. doi: 10.1016/0092-8674(82)90452-4.
6
Beta + thalassemia: aberrant splicing results from a single point mutation in an intron.β+地中海贫血：异常剪接由内含子中的单个点突变引起。
Cell. 1981 Dec;27(2 Pt 1):289-98. doi: 10.1016/0092-8674(81)90412-8.
7
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. doi: 10.1093/nar/11.5.1475.
8
beta-Thalassemia in Chinese: use of in vivo RNA analysis and oligonucleotide hybridization in systematic characterization of molecular defects.中国的β地中海贫血：体内RNA分析和寡核苷酸杂交在分子缺陷系统表征中的应用
Proc Natl Acad Sci U S A. 1984 May;81(9):2821-5. doi: 10.1073/pnas.81.9.2821.
9
beta-Thalassemia in American Blacks: novel mutations in the "TATA" box and an acceptor splice site.美国黑人中的β地中海贫血：“TATA”框和一个剪接受体位点的新突变
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1154-8. doi: 10.1073/pnas.81.4.1154.
10
Splicing of adenovirus RNA in a cell-free transcription system.腺病毒RNA在无细胞转录系统中的剪接
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5230-4. doi: 10.1073/pnas.80.17.5230.