Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Key Laboratory for Information Agriculture, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, China.
Cell Rep. 2024 Apr 23;43(4):113985. doi: 10.1016/j.celrep.2024.113985. Epub 2024 Mar 21.
Emerging evidence suggests a beneficial role of rhizobacteria in ameliorating plant disease resistance in an environment-friendly way. In this study, we characterize a rhizobacterium, Bacillus cereus NJ01, that enhances bacterial pathogen resistance in rice and Arabidopsis. Transcriptome analyses show that root inoculation of NJ01 induces the expression of salicylic acid (SA)- and abscisic acid (ABA)-related genes in Arabidopsis leaves. Genetic evidence showed that EDS1, PAD4, and WRKY18 are required for B. cereus NJ01-induced bacterial resistance. An EDS1-PAD4 complex interacts with WRKY18 and enhances its DNA binding activity. WRKY18 directly binds to the W box in the promoter region of the SA biosynthesis gene ICS1 and ABA biosynthesis genes NCED3 and NCED5 and contributes to the NJ01-induced bacterial resistance. Taken together, our findings indicate a role of the EDS1/PAD4-WRKY18 complex in rhizobacteria-induced disease resistance.
新出现的证据表明，根际细菌以一种环保的方式在改善植物疾病抗性方面发挥着有益的作用。在这项研究中，我们对一种能够增强水稻和拟南芥细菌病原体抗性的根际细菌解淀粉芽孢杆菌 NJ01 进行了描述。转录组分析表明，NJ01 根际接种诱导拟南芥叶片中水杨酸（SA）和脱落酸（ABA）相关基因的表达。遗传证据表明，EDS1、PAD4 和 WRKY18 是解淀粉芽孢杆菌 NJ01 诱导的细菌抗性所必需的。一个 EDS1-PAD4 复合物与 WRKY18 相互作用，并增强其 DNA 结合活性。WRKY18 直接结合到 SA 生物合成基因 ICS1 和 ABA 生物合成基因 NCED3 和 NCED5 启动子区域的 W 框上，并有助于 NJ01 诱导的细菌抗性。总之，我们的研究结果表明，EDS1/PAD4-WRKY18 复合物在根际细菌诱导的疾病抗性中发挥作用。
