Key Laboratory of Optoelectronics Technology, Beijing University of Technology, Ministry of Education, Beijing, China; Key Laboratory of Optoelectronic Materials and Devices, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China.
Key Laboratory of Optoelectronics Technology, Beijing University of Technology, Ministry of Education, Beijing, China; Key Laboratory of Optoelectronic Materials and Devices, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, China.
Biosens Bioelectron. 2024 Jun 15;254:116220. doi: 10.1016/j.bios.2024.116220. Epub 2024 Mar 16.
As Alzheimer's disease prevalence continues to rise, there is an increasing demand for efficient on-chip biosensors capable of early biomarker detection. This study presents a novel biosensor chip leveraging vertical cavity surface emitting laser (VCSEL) technology, with Parylene C serving as the antibody coupling layer and utilizing a streamlined one-step antibody modification method. Integration of Parylene C enhances chip sensitivity from 34.28 μW/RIU to 40.32 μW/RIU. Moreover, post-testing removal of Parylene C enables chip reusability without significant alteration of results. The sensor demonstrates effective detection of Aβ, an Alzheimer's biomarker, exhibiting a linear range of 1-200 ng/mL and a detection limit of 0.26 ng/mL. These findings underscore the reusability and reliability of the ultrathin Parylene C-based VCSEL biosensor chip, highlighting its potential for point-of-care Alzheimer's disease diagnosis.
随着阿尔茨海默病患病率的不断上升，人们对能够进行早期生物标志物检测的高效片上生物传感器的需求日益增加。本研究提出了一种利用垂直腔面发射激光器（VCSEL）技术的新型生物传感器芯片，其中聚对二甲苯 C 用作抗体偶联层，并采用简化的一步法抗体修饰方法。聚对二甲苯 C 的集成将芯片灵敏度从 34.28 μW/RIU 提高到 40.32 μW/RIU。此外，测试后去除聚对二甲苯 C 可实现芯片的重复使用，而不会对结果产生重大影响。该传感器能够有效检测阿尔茨海默病生物标志物 Aβ，具有 1-200 ng/mL 的线性范围和 0.26 ng/mL 的检测限。这些发现突出了基于超薄聚对二甲苯 C 的 VCSEL 生物传感器芯片的可重复使用性和可靠性，强调了其在即时护理阿尔茨海默病诊断中的潜力。
