Key Laboratory of Optoelectronic Materials and Devices, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China; College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, China.
School of Electronic and Control Engineering, Chang'an University, Xi'an, China.
Talanta. 2024 Aug 1;275:126111. doi: 10.1016/j.talanta.2024.126111. Epub 2024 Apr 16.
Sensitive, accurate, and straightforward biosensors are pivotal in the battle against Alzheimer's disease, particularly in light of the escalating patient population. These biosensors enable early adjunctive diagnosis, thereby facilitating prompt intervention, alleviating socioeconomic burdens, and preserving individual well-being. In this study, we introduce the development of a highly sensitive add-drop dual-microring resonant microfluidic sensing chip boasting a sensitivity of 188.11 nm/RIU, marking a significant 20.7% enhancement over single microring systems. Leveraging ultra-thin Parylene C for streamlined antibody immobilization and non-destructive removal, this platform facilitates the precise quantification of the Alzheimer's disease biomarker Aβ. Employing an immune sensing strategy that amplifies and captures antigen signals using Au-labeled antibodies, we achieve an exceptional limit of detection of 9.02 pg/mL. The designed microring-based microfluidic biosensor chip exhibits outstanding specificity and sensitivity for Aβ in serum samples, offering a promising avenue for the early adjunctive diagnosis of Alzheimer's disease.
敏感、准确且直接的生物传感器在对抗阿尔茨海默病的斗争中至关重要，尤其是考虑到患者人数不断增加的情况。这些生物传感器能够进行早期辅助诊断，从而促进及时干预、减轻社会经济负担并保护个人福祉。在这项研究中，我们介绍了一种高度敏感的添加-删除双微环谐振微流控传感芯片的开发，其灵敏度为 188.11nm/RIU，比单微环系统提高了 20.7%。该平台利用超薄膜聚对二甲苯实现了抗体的流线型固定和无损去除，从而精确地定量了阿尔茨海默病生物标志物 Aβ。我们采用了一种免疫传感策略，使用 Au 标记的抗体来放大和捕获抗原信号，实现了 9.02pg/mL 的卓越检测限。设计的基于微环的微流控生物传感器芯片在血清样本中对 Aβ 表现出出色的特异性和灵敏度，为阿尔茨海默病的早期辅助诊断提供了有前途的途径。
