Zhang Zhuang, Li Deyong, Zhou Changhui, Huang Xiaoshan, Chen Yantong, Wang Shijie, Liu Guoqiang
Guangdong Engineering Research Center of Water Treatment Processes and Materials, Guangdong Key Laboratory of Environmental Pollution and Health, and School of Environment and Climate, Jinan University, Guangzhou 510632, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China.
Guangdong Engineering Research Center of Water Treatment Processes and Materials, Guangdong Key Laboratory of Environmental Pollution and Health, and School of Environment and Climate, Jinan University, Guangzhou 510632, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China.
Water Res. 2024 May 15;255:121491. doi: 10.1016/j.watres.2024.121491. Epub 2024 Mar 19.
Pre-capturing organics in municipal wastewater for biogas production, combined with Anammox-based nitrogen removal process, improves the sustainability of sewage treatment. Thus, enhancing nitrogen removal via Anammox in mainstream wastewater treatment becomes very crucial. In present study, a three-stage anoxic/oxic (AO) biofilm process with intermittent aeration was designed to strengthen partial nitrification/denitrification coupling Anammox (PNA/PDA) in treatment of low C/N wastewater, which contained chemical oxygen demand (COD) of 79.8 mg/L and total inorganic nitrogen (TIN) of 58.9 mg/L. With a hydraulic retention time of 8.0 h, the process successfully reduced TIN to 10.6 mg/L, achieving a nitrogen removal efficiency of 83.3 %. The 1 anoxic zone accounted for 32.0 % TIN removal, with 10.3 % by denitrification and 21.7 % by PDA, meanwhile, the 2 and 3 anoxic zones contributed 19.4 % and 4.5 % of TIN removal, primarily achieved through PDA (including endogenous PD coupling Anammox). The 1 and 2 intermittent zones accounted for 27.2 % and 17.0 % of TIN removal, respectively, with 13.7 %-21.3 % by PNA and 3.2 %-5.3 % by PDA. Although this process did not pursue nitrite accumulation in any zone (< 1.5 mg-N/L), PNA and PDA accounted for 35.1 % and 52.1 % of TIN removal, respectively. Only 0.21 % of removed TIN was released as nitrous oxide. The AnAOB of Candidatus Brocadia was enriched in each zone, with a relative abundance of 0.66 %-2.29 %. In intermittent zones, NOB had been partially suppressed (AOB/NOB = 0.73-0.88), mainly due to intermittent aeration and effective nitrite utilization by AnAOB since its population size was much greater than NOB. Present study indicated that the three-stage AO biofilm process with intermittent aeration could enhance nitrogen removal via PNA and PDA with a low NO emission factor.
在城市污水中预捕获有机物用于沼气生产，结合基于厌氧氨氧化的脱氮工艺，可提高污水处理的可持续性。因此，在主流污水处理中通过厌氧氨氧化提高脱氮效率变得至关重要。在本研究中，设计了一种具有间歇曝气的三段式缺氧/好氧（AO）生物膜工艺，以强化处理低C/N污水（化学需氧量（COD）为79.8 mg/L，总无机氮（TIN）为58.9 mg/L）中的部分硝化/反硝化耦合厌氧氨氧化（PNA/PDA）。在水力停留时间为8.0 h的情况下，该工艺成功将TIN降低至10.6 mg/L，脱氮效率达到83.3%。第一缺氧区占TIN去除量的32.0%，其中反硝化去除10.3%，PDA去除21.7%，同时，第二和第三缺氧区分别贡献了TIN去除量的19.4%和4.5%，主要通过PDA实现（包括内源性PD耦合厌氧氨氧化）。第一和第二间歇区分别占TIN去除量的27.2%和17.0%，其中PNA去除13.7%-21.3%，PDA去除3.2%-5.3%。尽管该工艺在任何区域都未追求亚硝酸盐积累（<1.5 mg-N/L），但PNA和PDA分别占TIN去除量的35.1%和52.1%。仅0.21%的去除TIN以氧化亚氮形式释放。“Candidatus Brocadia”属的厌氧氨氧化菌在每个区域均得到富集，相对丰度为0.66%-2.29%。在间歇区，亚硝酸盐氧化菌（NOB）已被部分抑制（氨氧化菌（AOB）/NOB = 0.73-0.88），主要原因是间歇曝气以及厌氧氨氧化菌对亚硝酸盐的有效利用，因为其种群规模远大于NOB。本研究表明，具有间歇曝气的三段式AO生物膜工艺可通过PNA和PDA提高脱氮效率，且氧化亚氮排放因子较低。
