Li Deyong, Wang Shijie, Liu Guoqiang, Zeng Eddy Y
School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China.
School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China; Shenzhen Guangming Water and Environment Co., Ltd., Shenzhen 518107, China.
Bioresour Technol. 2025 Mar;419:132096. doi: 10.1016/j.biortech.2025.132096. Epub 2025 Jan 17.
Efficient nitrogen removal after organic capture is challenging through conventional nitrification-denitrification process. Two biofilm-based anoxic/oxic reactors, with a single intermittent zone (R1) or dual intermittent zones (R2), were compared in treating carbon-limited wastewater. Intermittent aeration integrated partial nitrification-anammox (PNA), partial denitrification-anammox (PDA), and denitrification, with anammox-related pathways contributing over 75% nitrogen removal in both reactors. As nitrogen loading rate increased from 0.14 to 0.19 kg-N m day, nitrogen removal efficiency in R1 dropped from 74.3% to 46.0%, while R2 maintained 76.6% removal at low HRT of 6 h. The dual intermittent aeration strategy improved nitrogen removal capacity by enhancing PNA in the first intermittent zone and reducing effluent fluctuation in the second. Anammox bacteria (Candidatus Brocadia, relative abundance: 0.95-2.48%) were enriched across all zones, supporting efficient PNA and PDA. These findings suggested that dual intermittent aeration enhanced anammox in pre-anoxic processes for carbon limited wastewater treatment.
通过传统的硝化-反硝化工艺，在有机物质捕获后实现高效的氮去除具有挑战性。比较了两个基于生物膜的缺氧/好氧反应器，一个具有单个间歇区（R1），另一个具有双间歇区（R2），用于处理碳限制废水。间歇曝气整合了部分硝化-厌氧氨氧化（PNA）、部分反硝化-厌氧氨氧化（PDA）和反硝化过程，在两个反应器中，与厌氧氨氧化相关的途径贡献了超过75%的氮去除率。随着氮负荷率从0.14增加到0.19 kg-N/m²·天，R1中的氮去除效率从74.3%下降到46.0%，而R2在6小时的低水力停留时间下保持了76.6%的去除率。双间歇曝气策略通过增强第一个间歇区的PNA和减少第二个间歇区的出水波动，提高了氮去除能力。厌氧氨氧化细菌（Candidatus Brocadia，相对丰度：0.95-2.48%）在所有区域都得到了富集，支持了高效的PNA和PDA。这些发现表明，双间歇曝气增强了碳限制废水处理的预缺氧过程中的厌氧氨氧化。
