State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Lancaster Environment Centre (LEC), Lancaster University, Lancaster LA1 4YQ, UK.
Ecotoxicol Environ Saf. 2024 Apr 15;275:116235. doi: 10.1016/j.ecoenv.2024.116235. Epub 2024 Mar 22.
Many gastrointestinal simulation methods have been used to predict bioavailability, but the suitability of different methods for the same metal(loid)s varies widely, which inevitably affects the accuracy of human health risk assessment. Arsenic is a common and important contaminant in many contaminated land situations. It can be readily absorbed and has teratogenic and mutagenic toxicity. Therefore, in this study, four the most commonly used in vitro simulation methods (the Physiologically Based Extraction Test (PBET), In Vitro Gastrointestinal Method (IVG), Soluble Bioavailability Research Consortium (SBRC), the Unified BARGE Method (UBM)) were tested against an in vivo animal live model, to evaluate their effectiveness for the prediction of soil As bioavailability in 10 industrially contaminated soils. The soil As relative bioavailability (RBA) varied between 15% and 68% in the different soils. As bioaccessibility differed between the 4 gastro-intestinal simulation methods. Gastric phase of UBM (UBMG) predicted As relative bioavailability the best of the 4 assays (R = 0.81). This study provides theoretical and technical support to refine human health risk assessment of As in soils from urban industrial legacy contaminated sites.
许多胃肠道模拟方法已被用于预测生物利用度，但不同方法对同一种金属（类）的适用性差异很大，这不可避免地影响了人类健康风险评估的准确性。砷是许多污染土地情况下常见且重要的污染物。它很容易被吸收，具有致畸和致突变毒性。因此，在这项研究中，我们对四种最常用的体外模拟方法（生理基础提取测试 (PBET)、体外胃肠道方法 (IVG)、可溶生物利用度研究联合会 (SBRC)、统一 BARGE 方法 (UBM)) 进行了测试，以评估它们在预测 10 种工业污染土壤中土壤砷生物利用度方面的有效性。不同土壤中土壤砷的相对生物利用率 (RBA) 介于 15%和 68%之间。4 种胃肠道模拟方法之间的砷生物可给性存在差异。UBM 的胃相 (UBMG) 对 4 种测定方法中砷的相对生物利用率预测最好（R = 0.81）。这项研究为细化城市工业遗留污染场地土壤中砷的人类健康风险评估提供了理论和技术支持。
