犬肾细胞癌中 CD3、FoxP3 和 granzyme B 免疫染色的定量分析。
Quantification of CD3, FoxP3, and granzyme B immunostaining in canine renal cell carcinoma.
机构信息
Department of Clinical Studies and Advanced Medicine, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
Department of Pathobiology, University of Pennsylvania, School of Veterinary Medicine, 3900 Spruce Street, Philadelphia, PA 19104, USA.
出版信息
Vet Immunol Immunopathol. 2024 May;271:110741. doi: 10.1016/j.vetimm.2024.110741. Epub 2024 Mar 16.
Tumor-infiltrating lymphocyte (TIL) density plays an important role in anti-tumor immunity and is associated with patient outcome in various human and canine malignancies. As a first assessment of the immune landscape of the tumor microenvironment in canine renal cell carcinoma (RCC), we retrospectively analyzed clinical data and quantified CD3, FoxP3, and granzyme B immunostaining in formalin-fixed paraffin-embedded tumor samples from 16 dogs diagnosed with renal cell carcinoma treated with ureteronephrectomy. Cell density was low for all markers evaluated. Increased numbers of intratumoral FoxP3 labelled (+) cells, as well as decreased granzyme B+: FoxP3+ TIL ratio, were associated with poor patient outcomes. Our initial study of canine RCC reveals that these tumors are immunologically cold and Tregs may play an important role in immune evasion.
肿瘤浸润淋巴细胞（TIL）密度在抗肿瘤免疫中起着重要作用，并与各种人类和犬科恶性肿瘤患者的预后相关。作为对犬肾细胞癌（RCC）肿瘤微环境免疫景观的首次评估，我们回顾性分析了 16 只接受输尿管肾切除术治疗的诊断为肾细胞癌的犬的临床数据，并对福尔马林固定石蜡包埋肿瘤样本中的 CD3、FoxP3 和颗粒酶 B 免疫染色进行了定量。所有评估的标志物的细胞密度均较低。肿瘤内 FoxP3 标记 (+) 细胞数量增加，以及颗粒酶 B+：FoxP3+ TIL 比值降低与患者预后不良相关。我们对犬肾细胞癌的初步研究表明，这些肿瘤具有免疫冷性，调节性 T 细胞（Tregs）可能在免疫逃逸中发挥重要作用。
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