Inoue Akiko, Maeda Shingo, Kinoshita Ryohei, Tsuboi Masaya, Yonezawa Tomohiro, Matsuki Naoaki
Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Vet Immunol Immunopathol. 2017 Aug;190:53-56. doi: 10.1016/j.vetimm.2017.07.001. Epub 2017 Jul 6.
Although tumor-infiltrating lymphocytes (TILs) play a key role in anti-tumor immunity, their involvement in canine transitional cell carcinoma (TCC) is not well-documented. The objective of this study was to investigate the association between TIL number and prognosis in dogs with urinary bladder TCC. Immunohistochemical analysis of CD3 and granzyme B was performed using canine TCC (n=32) and normal bladder (n=10) tissues. The numbers of CD3 and granzyme B cells located in peritumoral stroma of canine TCC were significantly higher than those in normal controls. In TCC cases, the number of CD3 TILs was not significantly related to prognosis, whereas the abundant granzyme B TILs were associated with favorable outcome. Since granzyme B TILs were not associated with the tumor stage, the presence of granzyme B TILs may be an independent prognostic factor. These results suggest that granzyme B TILs play a role in anti-tumor immunity and inhibit tumor progression in canine TCC.
尽管肿瘤浸润淋巴细胞（TILs）在抗肿瘤免疫中发挥关键作用，但其在犬移行细胞癌（TCC）中的作用尚无充分文献记载。本研究的目的是调查膀胱TCC患犬中TIL数量与预后之间的关联。使用犬TCC组织（n = 32）和正常膀胱组织（n = 10）进行CD3和颗粒酶B的免疫组织化学分析。位于犬TCC肿瘤周围基质中的CD3和颗粒酶B细胞数量显著高于正常对照。在TCC病例中，CD3 TILs数量与预后无显著相关性，而丰富的颗粒酶B TILs与良好预后相关。由于颗粒酶B TILs与肿瘤分期无关，颗粒酶B TILs的存在可能是一个独立的预后因素。这些结果表明，颗粒酶B TILs在犬TCC的抗肿瘤免疫中发挥作用并抑制肿瘤进展。
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