一种感染白首乌的新型马铃薯卷叶病毒的全基因组序列

Complete genome sequence of a novel polerovirus infecting Cynanchum rostellatum.

作者信息

Tokuda Ryosuke, Watanabe Kiyoto, Koinuma Hiroaki, Okano Yukari, Nijo Takamichi, Yamamoto Toya, Suzuki Masato, Maejima Kensaku, Namba Shigetou, Yamaji Yasuyuki

机构信息

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.

Faculty of Food and Agricultural Sciences, Fukushima University, 1 Kanayagawa, Fukushima, Fukushima, 960-1296, Japan.

出版信息

Arch Virol. 2023 Jan 9;168(2):57. doi: 10.1007/s00705-022-05625-1.

DOI:10.1007/s00705-022-05625-1

PMID:36617596

Abstract

We detected a virus-like sequence in Cynanchum rostellatum leaves showing yellow mottle symptoms, found in Tokyo, Japan. RNA-Seq analysis revealed that the complete nucleotide sequence of the virus genome was 5,878 nucleotides in length and that it contained seven open reading frames (ORFs) specific to members of the genus Polerovirus. Accordingly, phylogenetic analysis revealed that the virus clustered with poleroviruses in the family Solemoviridae. The amino acid sequence identity values obtained by comparison of the deduced proteins of this virus and those of known members of the genus Polerovirus were lower than 90%, which is the species demarcation criterion of the taxon. The results indicate that this virus is a novel member of the genus Polerovirus, for which the name "cynanchum yellow mottle-associated virus" is proposed.

摘要

我们在日本东京发现的呈现黄色斑驳症状的柳叶白前叶片中检测到一种病毒样序列。RNA测序分析表明，该病毒基因组的完整核苷酸序列长度为5878个核苷酸，并且包含七个专属于粉虱传双生病毒属成员的开放阅读框（ORF）。因此，系统发育分析表明，该病毒与独脚金病毒科中的粉虱传双生病毒聚集在一起。通过比较该病毒推导的蛋白质与粉虱传双生病毒属已知成员的蛋白质所获得的氨基酸序列同一性值低于90%，这是该分类单元的种划分标准。结果表明，这种病毒是粉虱传双生病毒属的一个新成员，为此提出了“柳叶白前黄色斑驳相关病毒”这一名称。