İnanir Merve, Uçar Esra, Tüzün Burak, Eruygur Nuraniye, Ataş Mehmet, Akpulat Hüseyin Aşkın
Department of Pharmacology, Faculty of Pharmacy, Sivas Cumhuriyet University, Sivas, Turkey.
Department of Medicinal and Aromatic Plant, Vocational School, Sivas Cumhuriyet University, Sivas, Turkey.
Int J Biol Macromol. 2024 May;266(Pt 2):130943. doi: 10.1016/j.ijbiomac.2024.130943. Epub 2024 Mar 22.
The aim of this study is to evaluate and compare the biological properties of different extracts (methanol, ethanol, and water) obtained from Gypsophila eriocalyx (G. eriocalyx), a medicinal plant traditionally used in Turkey. The components of different extracts were defined using the GC-MS method. The effects of G. eriocalyx extracts on cell proliferation, apoptosis, and cell cycle arrest in MDA-MB-231 breast cancer as well as in vitro antioxidant, enzyme inhibition, and antimicrobial activities were investigated. In accordance with the results obtained, although ethanol and methanol extracts of G. eriocalyx show higher antioxidant activity than G. eriocalyx water extract, enzyme inhibition activities of the extracts were not found to be significant compared to the reference drug. The methanol and ethanol extract of G. eriocalyx exhibited moderate antimicrobial activity against Staphylococcus aureus and methanol extract showed significant antimicrobial activity against Bacillus cereus. In addition, both extracts significantly inhibited cell viability in a dose-dependent manner in breast cancer cells. The cell growth inhibition by methanol and ethanol extracts induced S phase cell-cycle arrest and apoptosis in MDA-MB-231 cells. Lastly, in order to compare the activities of the chemicals found in Gypsophila eriocalyx plant extract, their activities against various proteins that are breast cancer protein (PDB ID:1A52 and 1JNX), antioxidant protein (PDB ID: 1HD2), AChE enzyme protein (PDB ID: 4M0E), BChE enzyme protein (PDB ID: 5NN0), and Escherichia coli protein (PDB ID: 4PRV)were compared. Then, ADME/T analysis calculations were made to examine the effects of molecules with high activity on human metabolism. Eventually, G. eriocalyx is thought to be a potent therapeutic herb that can be considered as an alternative and functional therapy for the management of diseases of a progressive nature related to oxidative damage such as infection, diabetes, cancer, and Alzheimer's disease.
本研究的目的是评估和比较从土耳其传统药用植物细叶丝石竹（Gypsophila eriocalyx）中获得的不同提取物（甲醇、乙醇和水）的生物学特性。使用气相色谱-质谱联用（GC-MS）方法确定不同提取物的成分。研究了细叶丝石竹提取物对MDA-MB-231乳腺癌细胞增殖、凋亡和细胞周期阻滞的影响，以及体外抗氧化、酶抑制和抗菌活性。根据所得结果，虽然细叶丝石竹的乙醇和甲醇提取物比细叶丝石竹水提取物表现出更高的抗氧化活性，但与参考药物相比，提取物的酶抑制活性并不显著。细叶丝石竹的甲醇和乙醇提取物对金黄色葡萄球菌表现出中等抗菌活性，甲醇提取物对蜡样芽孢杆菌表现出显著抗菌活性。此外，两种提取物均以剂量依赖性方式显著抑制乳腺癌细胞的活力。甲醇和乙醇提取物对细胞生长的抑制诱导了MDA-MB-231细胞的S期细胞周期阻滞和凋亡。最后，为了比较细叶丝石竹植物提取物中发现的化学物质的活性，比较了它们对各种蛋白质的活性，这些蛋白质包括乳腺癌蛋白（PDB ID:1A52和1JNX）、抗氧化蛋白（PDB ID: 1HD2）、乙酰胆碱酯酶（AChE）蛋白（PDB ID: 4M0E）、丁酰胆碱酯酶（BChE）蛋白（PDB ID: 5NN0）和大肠杆菌蛋白（PDB ID: 4PRV）。然后，进行了药物代谢及药物动力学/毒性预测（ADME/T）分析计算，以研究具有高活性的分子对人体代谢的影响。最终，细叶丝石竹被认为是一种有效的治疗性草药，可被视为对与氧化损伤相关的进行性疾病（如感染、糖尿病、癌症和阿尔茨海默病）进行管理的替代和功能性疗法。
