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一种新型GXYX粗脂肽对小鼠鼠伤寒沙门氏菌感染的影响。

Effects of a novel GXYX crude lipopeptide against serovar Typhimurium infection in mice.

作者信息

Zhang Jingya, Wu Yifan, Li Wei, Xie Honglin, Li Jingyan, Miao Yongqiang, Yang Zengqi, Zhou Yefei, Wang Xinglong

机构信息

College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.

College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China.

出版信息

Heliyon. 2024 Mar 15;10(6):e28219. doi: 10.1016/j.heliyon.2024.e28219. eCollection 2024 Mar 30.

DOI:10.1016/j.heliyon.2024.e28219
PMID:38524560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10958701/
Abstract

The increased rate of antibiotic resistance strongly limits the resolution of enterica serovar Typhimurium (S. Typhimurium) infection. Therefore, new strategies to control bacterial infections are urgently needed. and its metabolites are desirable antibacterial agents. Here, we aimed to evaluate the antibacterial activity of the novel strain GXYX (No: PRJNA940956) crude lipopeptide against . Typhimurium. In vitro, GXYX crude lipopeptides affected . Typhimurium biofilm formation and swimming and attenuated the adhesion and invasion abilities of . Typhimurium toward BHK-21 cells; in addition, it inhibited the mRNA expression of the , , , and genes, which are related to the adhesion and invasion ability of . Typhimurium. In vivo, pretreatment with GXYX crude lipopeptide via intragastric administration improved the survival rate by 30%, which was related to reductions in organ bacterial loads and clinical signs in mice. Intragastric administration of GXYX crude lipopeptide significantly downregulated the mRNA levels of TNF-α, IL-1β, IL-12 and IL-6 in response to . Typhimurium-induced inflammation compared with intraperitoneal injection. Moreover, it significantly improved the intestinal barrier-related gene (ZO-1, claudin-1, occludin-1) mRNA levels in intestinal tissue damaged by S. Typhimurium infection. In conclusion, GXYX crude lipopeptides were effective at reducing . Typhimurium colonization, laying a foundation for the further development of novel antibacterial agents.

摘要

抗生素耐药率的上升严重限制了鼠伤寒沙门氏菌感染的解决。因此，迫切需要控制细菌感染的新策略。其代谢产物是理想的抗菌剂。在这里，我们旨在评估新型菌株GXYX（编号：PRJNA940956）粗脂肽对鼠伤寒沙门氏菌的抗菌活性。在体外，GXYX粗脂肽影响鼠伤寒沙门氏菌生物膜的形成和游动，并减弱鼠伤寒沙门氏菌对BHK-21细胞的黏附和侵袭能力；此外，它还抑制了与鼠伤寒沙门氏菌黏附和侵袭能力相关的、、、和基因的mRNA表达。在体内，通过胃内给药预先使用GXYX粗脂肽可使存活率提高30%，这与小鼠器官细菌载量和临床症状的减少有关。与腹腔注射相比，胃内给药GXYX粗脂肽可显著下调鼠伤寒沙门氏菌诱导炎症反应中TNF-α、IL-1β、IL-12和IL-6的mRNA水平。此外，它还显著提高了受鼠伤寒沙门氏菌感染损伤的肠道组织中肠道屏障相关基因（ZO-1、claudin-1、occludin-1）的mRNA水平。总之，GXYX粗脂肽在减少鼠伤寒沙门氏菌定植方面是有效的，为新型抗菌剂的进一步开发奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/0de4f2831ef4/mmcfigs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/b92c4d82ebf2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/0a9c04cf7f30/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/f9bcff4f55a5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/11e188402ecf/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/615a10489a58/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/45be74b76c17/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/17df7c7bd69d/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/ddfde6ece374/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/418084f00c1f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/fa874b60eb31/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/0de4f2831ef4/mmcfigs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/b92c4d82ebf2/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/0a9c04cf7f30/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/f9bcff4f55a5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/11e188402ecf/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/615a10489a58/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/45be74b76c17/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/17df7c7bd69d/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/ddfde6ece374/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/418084f00c1f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/fa874b60eb31/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1142/10958701/0de4f2831ef4/mmcfigs2.jpg

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