Abo-Hammam Rana H, Salah Mohammed, Shabayek Sarah, Hanora Amro, Zakeer Samira, Khattab Randa H
Forensic toxicologist and narcotics expert, Ministry of Justice, Tanta, Egypt.
Department of Microbiology and Immunology, Faculty of pharmacy, Port-Said University, Port-Said, Egypt.
AIMS Microbiol. 2024 Feb 20;10(1):148-160. doi: 10.3934/microbiol.2024008. eCollection 2024.
One of the most prevalent malignancies that significantly affects world health is colorectal cancer (CRC). While genetics are involved in a portion of CRC patients, most cases are sporadic. The microbiome composition could be a new source of tumor initiation and progression. This research was conducted to investigate the microbiota composition of CRC patients post colectomy at taxonomic and functional levels. Using a next-generation sequencing approach, using an Illumina Novaseq 6000, the fecal samples of 13 patients were analyzed and the obtained data was subjected to a bioinformatics analysis. The bacterial abundance and uniqueness varied in CRC patients alongside differences in bacterial counts between patients. , , , and were among the pro-cancerous microorganisms found. Concurrently, bacteria linked to CRC progression were detected that have been previously linked to metastasis and recurrence. At the same time, probiotic bacteria such as , , and increased in abundance after colectomies. Additionally, numerous pathways were deferentially enriched in CRC, which emerged from functional pathways based on bacterial shotgun data. CRC-specific microbiome signatures include an altered bacterial composition. Our research showed that microbial biomarkers could be more usefully employed to explore the link between gut microbiota and CRC using metagenomic techniques in the diagnosis, prognosis, and remission of CRC, thereby opening new avenues for CRC treatment.
结直肠癌（CRC）是严重影响全球健康的最常见恶性肿瘤之一。虽然一部分CRC患者与遗传因素有关，但大多数病例是散发性的。微生物群组成可能是肿瘤发生和进展的新来源。本研究旨在从分类学和功能水平调查CRC患者结肠切除术后的微生物群组成。采用下一代测序方法，使用Illumina Novaseq 6000对13例患者的粪便样本进行分析，并对获得的数据进行生物信息学分析。CRC患者的细菌丰度和独特性各不相同，患者之间的细菌计数也存在差异。在发现的促癌微生物中，有[具体微生物名称1]、[具体微生物名称2]、[具体微生物名称3]和[具体微生物名称4]。同时，检测到与CRC进展相关的细菌，这些细菌先前已与转移和复发相关联。与此同时，诸如[具体益生菌名称1]、[具体益生菌名称2]和[具体益生菌名称3]等益生菌在结肠切除术后丰度增加。此外，基于细菌鸟枪法数据的功能途径中出现了许多在CRC中差异富集的途径。CRC特异性微生物群特征包括细菌组成的改变。我们的研究表明，微生物生物标志物可更有效地用于利用宏基因组技术探索肠道微生物群与CRC在诊断、预后和缓解方面的联系，从而为CRC治疗开辟新途径。
