Chen Jianwei, Liu Zeyang, Wang Zixian, Zhang Xiuxiu, Zhang Yi, Zhan Zhen, Gong Xiaohua, Xu Tao
Bio-intelligent Manufacturing and Living Matter Bioprinting Center, Research Institute of Tsinghua University in Shenzhen, Tsinghua University, Shenzhen 518057, People's Republic of China.
Precision Medicine and Healthcare Research Center, Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen 518055, People's Republic of China.
Regen Biomater. 2024 Mar 5;11:rbae021. doi: 10.1093/rb/rbae021. eCollection 2024.
There are many instances of hollow-structure morphogenesis in the development of tissues. Thus, the fabrication of hollow structures in a simple, high-throughput and homogeneous manner with proper natural biomaterial combination is valuable for developmental studies and tissue engineering, while it is a significant challenge in biofabrication field. We present a novel method for the fabrication of a hollow cell module using a coaxial co-flow capillary microfluidic device. Sacrificial gelatin laden with cells in the inner layer and GelMa in the outer layer are used via a coaxial co-flow capillary microfluidic device to produce homogenous micro-beads. The overall and core sizes of core-shell microbeads were well controlled. When using human vein vascular endothelial cells to demonstrate how cells line the inner surface of core-shell beads, as the core liquifies, a hollow cell ball with asymmetric features is fabricated. After release from the GelMa shell, individual cell balls are obtained and deformed cell balls can self-recover. This platform paves way for complex hollow tissue modeling , and further modulation of matrix stiffness, curvature and biochemical composition to mimic microenvironments.
在组织发育过程中有许多中空结构形态发生的实例。因此，以简单、高通量且均匀的方式结合适当的天然生物材料制造中空结构，对于发育研究和组织工程具有重要价值，然而这在生物制造领域是一项重大挑战。我们提出了一种使用同轴共流毛细管微流控装置制造中空细胞模块的新方法。通过同轴共流毛细管微流控装置，在内层使用负载细胞的牺牲性明胶，在外层使用甲基丙烯酰化明胶来制备均匀的微珠。核壳微珠的整体尺寸和核心尺寸得到了很好的控制。当使用人静脉血管内皮细胞来展示细胞如何排列在核壳珠的内表面时，随着核心液化，会制造出具有不对称特征的中空细胞球。从甲基丙烯酰化明胶壳中释放后，可获得单个细胞球，且变形的细胞球能够自我恢复。该平台为复杂中空组织建模以及进一步调节基质刚度、曲率和生化组成以模拟微环境铺平了道路。
