Center for Phage Technology, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA.
Viruses. 2023 Sep 23;15(10):1985. doi: 10.3390/v15101985.
Positive-sense single-stranded RNA (ssRNA) bacteriophages (phages) were first isolated six decades ago. Since then, extensive research has been conducted on these ssRNA phages, particularly those infecting . With small genomes of typically 3-4 kb that usually encode four essential proteins, ssRNA phages employ a straightforward infectious cycle involving host adsorption, genome entry, genome replication, phage assembly, and host lysis. Recent advancements in metagenomics and transcriptomics have led to the identification of ~65,000 sequences from ssRNA phages, expanding our understanding of their prevalence and potential hosts. This review article illuminates significant investigations into ssRNA phages, with a focal point on their structural aspects, providing insights into the various stages of their infectious cycle.
六十年前，人们首次分离出了正链单链 RNA（ssRNA）噬菌体（phage）。自此，人们对这些 ssRNA 噬菌体进行了广泛的研究，尤其是那些感染的噬菌体。ssRNA 噬菌体基因组通常较小，只有 3-4kb，编码四种必需蛋白，它们采用简单的感染周期，包括宿主吸附、基因组进入、基因组复制、噬菌体组装和宿主裂解。宏基因组学和转录组学的最新进展已经从 ssRNA 噬菌体中鉴定出约 65000 个序列，这增加了我们对它们的普遍性和潜在宿主的理解。本文综述了 ssRNA 噬菌体的重要研究进展，重点介绍了它们的结构方面，深入了解了它们感染周期的各个阶段。
