SKL of Marine Food Processing & Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P. R. China.
National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, P. R. China.
J Agric Food Chem. 2024 Apr 3;72(13):7517-7532. doi: 10.1021/acs.jafc.3c05812. Epub 2024 Mar 25.
In this study, the molecular mechanisms of iron transport and homeostasis regulated by the Antarctic krill-derived heptapeptide-iron (LVDDHFL-iron) complex were explored. LVDDHFL-iron significantly increased the hemoglobin, serum iron, total iron binding capacity levels, and iron contents in the liver and spleen to normal levels, regulated the gene expressions of iron homeostasis, and enhanced antioxidant capacity in iron-deficiency anemia mice ( < 0.05). The results revealed that iron ions within LVDDHFL-iron can be transported the heme transporter and divalent metal transporter-1, and the absorption of LVDDHFL-iron involved receptor-mediated endocytosis. We also found that the transport of LVDDHFL-iron across cells phagocytosis was facilitated by the up-regulation of the high mobility group protein, heat shock protein β, and V-type proton ATPase subunit, accompanied by the regulatory mechanism of autophagy. These findings provided deeper understandings of the mechanism of LVDDHFL-iron facilitating iron absorption.
在这项研究中，探索了南极磷虾衍生七肽-铁（LVDDHFL-iron）复合物调节铁转运和稳态的分子机制。LVDDHFL-iron 显著提高了缺铁性贫血小鼠的血红蛋白、血清铁、总铁结合能力和肝脏、脾脏中铁含量至正常水平，调节铁稳态基因表达，增强了抗氧化能力（<0.05）。结果表明，LVDDHFL-iron 内的铁离子可以通过血红素转运蛋白和二价金属转运蛋白-1 进行转运，LVDDHFL-iron 的吸收涉及受体介导的内吞作用。我们还发现，LVDDHFL-iron 的跨细胞转运通过吞噬作用被高迁移率族蛋白、热休克蛋白β和 V 型质子 ATP 酶亚基的上调所促进，同时伴随着自噬的调节机制。这些发现为 LVDDHFL-iron 促进铁吸收的机制提供了更深入的理解。
